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GS60

Sigma-Aldrich

GST A1-1, Recombinant Human

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Synonym(s):
GST-epsilon, GST2, GSTA1-1, GTH1, glutathione S-transferase alpha 1
NACRES:
NA.26

biological source

human

Quality Level

100

recombinant

expressed in E. coli

Assay

>95% (SDS-PAGE)

form

frozen liquid

specific activity

21.04 units/mg protein (using spectrophotometric determination of 1-chloro-2,4-dinitrobenzene (CDNB) conjugation with reduced glutathione (1 mM) in 100 mM NaPO4 (pH 6.5) at room temperature.)

mol wt

23 kDa

concentration

2.0 mg/mL

storage temp.

−70°C

Gene Information

human ... GSTA1(2938)

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recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

>95% (SDS-PAGE)

assay

>95% (SDS-PAGE)

assay

95% (SDS-PAGE)

assay

>95% (SDS-PAGE)

form

frozen liquid

form

frozen liquid

form

frozen liquid

form

frozen liquid

specific activity

21.04 units/mg protein (using spectrophotometric determination of 1-chloro-2,4-dinitrobenzene (CDNB) conjugation with reduced glutathione (1 mM) in 100 mM NaPO4 (pH 6.5) at room temperature.)

specific activity

24.07 (using spectrophotometric determination of 1-fluoro-2,4-dinitrobenzene (FDNB) conjugation with reduced glutathione (1 mM) in 100 mM NaPO4 (pH 6.5) at room temperature.)

specific activity

8.8 units/mg protein (as assayed by the spectrophotometric determination of NADPH oxidation coupled to the glutathione peroxidase activity of GST T1-1 on cumene hydroperoxide (1.5 mM) in the presence of reduced glutathione (1 mM) in 100 mM NaPO4 (pH 7.0) at room temperature.)

specific activity

97.96 units/mg protein (using spectrophotometric determination of 1-chloro-2,4-dinitrobenzene (CDNB) conjugation with reduced glutathione (1 mM) in 100 mM NaPO4 (pH 6.5) at room temperature.)

mol wt

23 kDa

mol wt

23 kDa

mol wt

25 kDa

mol wt

26 kDa

Biochem/physiol Actions

Glutathione S-transferase alpha 1 (GSTA1) is an enzyme that in humans is encoded by the GSTA1 gene. Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. The GSTs are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
The GSTA1 gene encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. This action is an important step in detoxification of these compounds. This subfamily of enzymes has a particular role in protecting cells from reactive oxygen species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. This gene is located in a cluster of similar genes and pseudogenes on chromosome 6. Alternative splicing results in multiple transcript variants.

Storage and Stability

The enzyme should be used by the end-user customer within 1 year of receipt.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Glutathione S-transferase A1 (GSTA1) appears to be primarily involved in detoxification processes, but possible roles in lung cancer remain unclear. The objective of this study was to investigate the expression and function of GSTA1 in lung cancer cells. Real-time PCR
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