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GUSA

Sigma-Aldrich

β-Glucuronidase Fluorescent Activity Detection Kit

Pricing and availability is not currently available.

usage

 kit sufficient for 200 standard tests

shipped in

dry ice

storage temp.

−20°C

Application

For quantitative assay of β-glucuronidase gene products in plant or animal cell lysates.

Features and Benefits

  • Fluorescent detection for quantitative and qualitative measurement
  • Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability
  • GUS does not interfere with plant cell function or viability

Other Notes

The substrate used in this kit is MU-GlcA (4-methylumbelliferyl β-D-glucuronide), a widely used fluorogenic substrate for determination of glucuronidase activity.

Kit Components Only

Product No.
Description

  • Detailed technical bulletin 1 ea

Kit Components Also Available Separately

Product No.
Description
SDS

  • 5× extraction buffer 25 mL

  • β-Glucuronidase 1000 U

  • M15084-MU 25 mg

  • 4-MU-GlcA 25 mg

  • Stop solution 100 mL

GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
Jefferson, R.A., et al.
The Embo Journal, 6, 390-3907 (1987)
An improved assay for beta-glucuronidase in transformed cells: methanol almost completely suppresses a putative endogenous beta-glucuronidase activity. .
Kosugi, S., et al.
Plant Science, 70, 133-140 (1990)
Current Protocols in Molecular Biology, 9-9 (1996)
R A Jefferson
Nature, 342(6251), 837-838 (1989-12-14)
The GUS reporter gene system is already a powerful tool for the assessment of gene activity in transgenic plants. Further developments may lead to routine in vivo analysis and fusion genetics.

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