GUSS

β-Glucuronidase Reporter Gene Staining Kit

• Synonym(s): β-Glucuronidase Staining Kit, Reporter Gene Kit, Staining Kit for Glucuronidase
• UNSPSC Code: 12161503
• NACRES: NA.32

Properties

• usage: kit sufficient for 100 histochemical assays
• Quality Level: 200
• technique(s): microbe id | staining: suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Kit is used to anaylze β glucuronidase (E.coli GUS gene) expression in transformed plants.

Application

The E. coli GUS gene is extensively used to analyze gene expression in transformed plants. Plants have low intrinsic GUS activity, and the E. coli gene is quite stable in plant cells. As a tag, GUS remains active at the N-terminus of fusion proteins.

Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.

Features and Benefits

• Histochemical staining of plant tissues expressing the E. coli GUS enzyme
• Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability
• GUS does not interfere with plant cell function or viability

Other Notes

The substrate used in this kit is X-GlcA (5-bromo-4-chloro-3-indolyl β-D-glucuronide) which results in an insoluble indigo-blue precipitate in transfected cells and tissues.

Kit Components Only

• 2× Fixation Buffer 25 mL
• 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98% 1 mL/vial
• Reagent A for gus staining kit 50 mL
• Reagent B for gus staining kit 200 μL
• Reagent C for gus staining kit 200 μL

Safety Information

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 11 - Combustible Solids