GUSS

β-Glucuronidase Reporter Gene Staining Kit

Properties

• Quality Level: 200
• usage: kit sufficient for 100 histochemical assays
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Kit is used to anaylze β glucuronidase (E.coli GUS gene) expression in transformed plants.

Application

The E. coli GUS gene is extensively used to analyze gene expression in transformed plants. Plants have low intrinsic GUS activity, and the E. coli gene is quite stable in plant cells. As a tag, GUS remains active at the N-terminus of fusion proteins.

Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.

Features and Benefits

• Histochemical staining of plant tissues expressing the E. coli GUS enzyme
• Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability
• GUS does not interfere with plant cell function or viability

Other Notes

The substrate used in this kit is X-GlcA (5-bromo-4-chloro-3-indolyl β-D-glucuronide) which results in an insoluble indigo-blue precipitate in transfected cells and tissues.

Kit Components Also Available Separately

• F2422 2× Fixation buffer 25 mL
• X-GlcA 1 mL/vial
• R6147 Reagent A 50 mL
• R6272 Reagent B 200 μL
• R6397 Reagent C 200 μL

Safety Information

• Hazard Statements: H412
• Precautionary Statements: P273 - P501
• RIDADR: NONH for all modes of transport
• WGK Germany: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable