H0135

Sigma-Aldrich

Hydrocortisone

γ-irradiated, powder, BioXtra, suitable for cell culture

Synonym(s):
11β,17α,21-Trihydroxypregn-4-ene-3,20-dione, Cortisol, Reichstein’s substance M, 4-Pregnene-11β,17α,21-triol-3,20-dione, 17-Hydroxycorticosterone, Kendall’s compound F
Empirical Formula (Hill Notation):
C21H30O5
CAS Number:
Molecular Weight:
362.46
Beilstein/REAXYS Number:
1354819
EC Number:
MDL number:
PubChem Substance ID:

biological source

synthetic (organic)

Quality Level

sterility

γ-irradiated

product line

BioXtra

assay

≥80% (HPLC)

form

powder

application(s)

cell culture | mammalian: suitable

mp

211-214 °C (lit.)

solubility

ethanol: 1 mg/mL, clear

shipped in

ambient

storage temp.

room temp

SMILES string

[H][C@@]12CCC3=CC(=O)CC[C@]3(C)[C@@]1([H])[C@@H](O)C[C@@]4(C)[C@@]2([H])CC[C@]4(O)C(=O)CO

InChI

1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1

InChI key

JYGXADMDTFJGBT-VWUMJDOOSA-N

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General description

Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.

Application

Hydrocortisone is used for the following applications:
  • Used in myogenic induction and identification
  • Used in long-term stromal cultures and methylcellulose cultures (one of the components in the culture medium)
  • Used in adipogenic differentiation (one of the components in adipogenic medium)
  • Used in cell culture: The human mammary epithelial cell MCF-10A cells were maintained in DMEM/F12 and supplemented with hydrocortisone along with other components
  • The SUM149 cell line was established from a primary IBC and grown in FBS supplemented with Ham′s F12 medium containing insulin and hydrocortisone
  • Used in differentiation assays of melanoma spheroid cells

Biochem/physiol Actions

Primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na2+ retention potency.

Physical form

powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw

Reconstitution

To prepare 50 μg/mL stock solution; add 1 mL absolute ethanol, gently swirl to dissolve, add 19 mL sterile medium while mixing.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

K L van Golen et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 5(9), 2511-2519 (1999-09-28)
Inflammatory breast cancer is a rapidly growing, distinct form of locally advanced breast cancer that carries a guarded prognosis. To identify the genes that contribute to this aggressive phenotype, we compared under- and overexpressed sequences in an inflammatory breast tumor...
Q L Hao et al.
Blood, 86(10), 3745-3753 (1995-11-15)
We present cell cycling and functional evidence that the CD34+CD38- immunophenotype can be used to define a rare and primitive subpopulation of progenitor cells in umbilical cord blood. CD34+CD38- cells comprise 0.05% +/- 0.08% of the mononuclear cells present in...
Andrej Jedinak et al.
International journal of oncology, 33(6), 1307-1313 (2008-11-21)
In spite of the global consumption of mushrooms, only two epidemiological studies demonstrated an inverse correlation between mushroom intake and the risk of cancer. Therefore, in the present study we evaluated whether extracts from edible mushrooms Agaricus bisporus (portabella), Flammulina...
Oscar K Lee et al.
Blood, 103(5), 1669-1675 (2003-10-25)
It is well accepted that umbilical cord blood has been a source for hematopoietic stem cells. However, controversy exists as to whether cord blood can serve as a source of mesenchymal stem cells, which can differentiate into cells of different...
Dong Fang et al.
Cancer research, 65(20), 9328-9337 (2005-10-19)
Recent studies suggest that cancer can arise from a cancer stem cell (CSC), a tumor-initiating cell that has properties similar to those of stem cells. CSCs have been identified in several malignancies, including those of blood, brain, and breast. Here...

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