H0161

Hyaluronic Acid Binding Protein bovine

lyophilized powder

• MDL number: MFCD02099073
• UNSPSC Code: 12352202
• NACRES: NA.56

Properties

• biological source: bovine
• Quality Level: 200
• form: lyophilized powder
• technique(s): immunofluorescence: suitable
• UniProt accession no.: P81282
• storage temp.: −20°C
• Gene Information: cow ... VCAN(282662)

Description

General description

Hyaluronic acid (HA) is a linear polysaccharide consisting of a repeating disaccharide of N-acetyl D-glucosamine (GlcNAc) and D-glucuronic acid (GlcA) with 1→4 interglycosidic linkages and these disaccharide repeating units are in turn linked by β (1→3) linkages. The number of repeating disaccharides in a HA molecule ranges from 2,000 to 25,000, which relates to a molecular mass of 106-107. The unique biological roles of HA is mainly due to the specific binding and interaction with HA-binding proteins also known as hyaladherins. Most hyaladherins is composed of a specific binding domain, also called a link module, which is composed of two α-helices and two antiparallel β-sheets.[1]

Karl Meyer and John Palmer first isolated hyaluronic acid (HA) from the vitreous of bovine eyes. They coined the name “hyaluronic acid” as a conjugation of two words, hyaloid (vitreous) and uronic acid. HA has an extended random coil structure in physiological solution. Due to its random-coil structure and high molecular weight (HMW), HA forms very viscose and elastic solution with a huge hydrodynamic volume.

Application

Hyaluronic Acid Binding Protein bovine may be used for incubation of specimens for immunofluorescence analysis of human megakaryocytes.[2]

Biochem/physiol Actions

Hyaluronic Acid Binding Protein (HABP) levels increases during oocyte maturation and it promotes cumulus expansion during in vitro maturation.[3]

In addition to being an important structural component of tissues in all vertebrates, HA is also associated with several biological functions including intracellular signaling.[1]

May be useful in hyaluronic acid binding or detection.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)