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H0262

Sigma-Aldrich

HAT Media Supplement (50×) Hybri-Max

γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

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Synonym(s):
HAT Supplement, Hypoxanthine-Aminopterin-Thymidin (HAT) media supplement

Quality Level

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

shipped in

dry ice

storage temp.

−20°C

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product line

BioXtra

product line

BioXtra

product line

BioXtra

product line

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

shipped in

dry ice

shipped in

-

shipped in

dry ice

shipped in

-

Application

HAT Media Supplement (50×) Hybri-Max has been used:
  • in the selection and validation of hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient clones
  • in the generation of mAbs (monoclonal antibodies)
  • in the luciferase-based bioassay (LUC) assay

Reconstitution

Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 0.4 μM aminopterin, 16 μM thymidine.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Repr. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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T1503
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25G
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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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