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H0262

Sigma-Aldrich

HAT Media Supplement (50×) Hybri-Max

lyophilized powder, suitable for hybridoma, BioXtra

Synonym(s):

HAT Supplement, Hypoxanthine-Aminopterin-Thymidin (HAT) media supplement

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

HAT Media Supplement (50×) Hybri-Max, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

Quality Level

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

shipped in

dry ice

storage temp.

−20°C

Application

HAT Media Supplement (50×) Hybri-Max has been used:
  • in the selection and validation of hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient clones
  • in the generation of mAbs (monoclonal antibodies)
  • in the luciferase-based bioassay (LUC) assay

Reconstitution

Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 0.4 μM aminopterin, 16 μM thymidine.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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