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H1136

Sigma-Aldrich

Hyaluronidase from Streptomyces hyalurolyticus

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Synonym(s):
Hyaluronate Lyase from Streptomyces hyalurolyticus
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

Streptomyces sp. (S. hyalurolyticus)

Quality Level

conjugate

conjugate (Glucosaminoglycan)

form

film (Clear)

packaging

ampule of 300 ×  units

storage temp.

−20°C

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This Item
H3631H3757H2126
Hyaluronidase from bovine testes Type VI-S, lyophilized powder, 3,000-15,000 units/mg solid

H3631

Hyaluronidase from bovine testes

Hyaluronidase from bovine testes Type VIII, lyophilized powder, 300-1,000 U/mg

H3757

Hyaluronidase from bovine testes

Hyaluronidase from sheep testes Type II, lyophilized powder, ≥300 units/mg solid

H2126

Hyaluronidase from sheep testes

form

film (Clear)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

conjugate

conjugate (Glucosaminoglycan)

conjugate

conjugate (Glucosaminoglycan)

conjugate

conjugate (Glucosaminoglycan)

conjugate

conjugate (Glucosaminoglycan)

packaging

ampule of 300 ×  units

packaging

-

packaging

-

packaging

-

General description

Hyaluronidase is widely present in nature. In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards.

Application

Hyaluronidase from Streptomyces hyalurolyticus has been used:
  • in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue
  • as a component of digestion solution for the derivation of germline stem cells from testicular tissue

Biochem/physiol Actions

Hyaluronate lyase cleaves hyaluronic acid at the β-D-GlcNAc-(1→4)-β-D-GlcA bond, yielding 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine tetra- and hexasaccharides. Unlike other hyaluronidases, this enzyme is specific for hyaluronic acid and is inactive with chondroitin and chondroitin sulfate.
Hyaluronidase is an endoglycosidase and performs its function along with exoglycosidases, like β-glucuronidase and β-N-acetyl glucosaminidase. Hyaluronidase regulates the hyaluronic acid turnover.

Unit Definition

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Tímea Váradi et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 60(8), 567-575 (2012-05-09)
Although trastuzumab is an efficient drug, primary and acquired resistance is a challenging problem. The authors have previously shown in mouse xenograft experiments that masking ErbB2 by hyaluronan leads to diminished binding of the antibody and consequent resistance. In the
Conversion of adult mouse unipotent germline stem cells into pluripotent stem cells
Ko K, et al.
Nature Protocols, 5(5), 921-921 (2010)
Wen Min Lau et al.
Cancer research, 74(9), 2630-2641 (2014-03-13)
The surface marker CD44 has been identified as one of several markers associated with cancer stem cells (CSC) in solid tumors, but its ubiquitous expression in many cell types, including hematopoietic cells, has hindered its use in targeting CSCs. In
Swathi Balaji et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(3), 868-881 (2016-12-03)
The cytokine IL-10 has potent antifibrotic effects in models of adult fibrosis, but the mechanisms of action are unclear. Here, we report a novel finding that IL-10 triggers a signal transducer and activator of transcription 3 (STAT3)-dependent signaling pathway that
CD44v8-10 is a cancer-specific marker for gastric cancer stem cells
Lau WM, et al.
Cancer Research, canres-ca2309 (2014)

Articles

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Protocols

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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