All Photos(1)



Hyaluronidase from Streptomyces hyalurolyticus

Hyaluronate Lyase from Streptomyces hyalurolyticus
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:

Quality Level


film (Clear)


ampule of 300 ×  units

storage temp.


Looking for similar products? Visit Product Comparison Guide

General description

Hyaluronidase is widely present in nature. In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards.


Hyaluronidase from Streptomyces hyalurolyticus has been used:
  • in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue
  • as a component of digestion solution for the derivation of germline stem cells from testicular tissue

Biochem/physiol Actions

Hyaluronate lyase cleaves hyaluronic acid at the β-D-GlcNAc-(1→4)-β-D-GlcA bond, yielding 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine tetra- and hexasaccharides. Unlike other hyaluronidases, this enzyme is specific for hyaluronic acid and is inactive with chondroitin and chondroitin sulfate.
Hyaluronidase is an endoglycosidase and performs its function along with exoglycosidases, like β-glucuronidase and β-N-acetyl glucosaminidase. Hyaluronidase regulates the hyaluronic acid turnover.

Unit Definition

One unit will cause a change in % transmittance at 600 nm of 0.330 per minute at pH 5.35 at 37 °C in a 2.0 mL reaction mixture (45 minute assay).

Storage Class Code

13 - Non Combustible Solids

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How should Product H1136, Hyaluronidase, be reconstituted?

    Prior to determining the activity of this enzyme, we reconstitute it in 20 mM sodium phosphate buffer, with 77 mM sodium chloride, 0.01% BSA, pH 7.0.

  4. What amount of product is present in the vial of Product H1136, Hyaluronidase?

    The dry weight of the product is not determined. The vial should contain a minimum of 300 units of enzyme.

  5. What is the molecular weight of Product H1136, Hyaluronidase?

    The molecular weight for hyaluronidase from Streptomyces hyalurolyticus has been reported to be 91,000 DALTONS as determined by gel filtration.

  6. Are any other components present in Product H1136, Hyaluronidase, besides the enzyme?

    No protein or BSA has been added as a stabilizer. The enzyme was lyophilized from 0.5 mM Tris buffer, pH 7.0.

  7. How is the activity of Product H1136, Hyaluronidase,determined?

    Sigma assays the enzyme against a USP hyaluronidase reference standard as described in USP XXII-NF XVII, 644 (1990).

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  11. I can’t see any material in the container. Is material present in the container?

    The product is lyophilized directly into the ampule and its appearance is a clear, thin film.

Swathi Balaji et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(3), 868-881 (2016-12-03)
The cytokine IL-10 has potent antifibrotic effects in models of adult fibrosis, but the mechanisms of action are unclear. Here, we report a novel finding that IL-10 triggers a signal transducer and activator of transcription 3 (STAT3)-dependent signaling pathway that...
Crystal structure of hyaluronidase, a major allergen of bee venom
Markovic H, et al.
Structure, 8(10), 1025-1035 (2000)
Connor C Gains et al.
Frontiers in bioengineering and biotechnology, 8, 700-700 (2020-08-09)
The Achilles tendon (AT) is comprised of three distinct sub-tendons bound together by the inter-subtendon matrix (ISTM). The interactions between sub-tendons will have important implications for AT function. The aim of this study was to investigate the extent to which...
Tímea Váradi et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 60(8), 567-575 (2012-05-09)
Although trastuzumab is an efficient drug, primary and acquired resistance is a challenging problem. The authors have previously shown in mouse xenograft experiments that masking ErbB2 by hyaluronan leads to diminished binding of the antibody and consequent resistance. In the...
Conversion of adult mouse unipotent germline stem cells into pluripotent stem cells
Ko K, et al.
Nature Protocols, 5(5), 921-921 (2010)


Carbohydrate Analysis III

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.


Enzymatic Assay of Hyaluronidase (

This procedure may be used for Hyaluronidase products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service