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H1404

Sigma-Aldrich

Hepatocyte Growth Factor human

HGF, recombinant, expressed in Baculovirus infected High-5 cells, suitable for cell culture

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Synonym(s):
HGF, Hepatopoietin A, Scatter Factor
EC Number:
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in Baculovirus infected High-5 cells

Assay

>95% (SDS-PAGE)

form

lyophilized powder

potency

1.0-20.0 ng/mL

quality

endotoxin tested

mol wt

~80 kDa

packaging

pkg of 5x5 μg
pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<1.0 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... HGF(3082)

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This Item
SRP6014F5542P1588
Sigma-Aldrich

Sigma-Aldrich

SRP6014

HGF human

recombinant

expressed in Baculovirus infected High-5 cells

recombinant

expressed in CHO cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

>95% (SDS-PAGE)

assay

≥97% (HPLC), ≥97% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

form

lyophilized powder

form

lyophilized

form

lyophilized powder

form

powder

potency

1.0-20.0 ng/mL

potency

-

potency

0.287 ng/mL

potency

-

mol wt

~80 kDa

mol wt

80.017 kDa

mol wt

15.5 kDa

mol wt

29 kDa

Biochem/physiol Actions

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

Other Notes

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

Physical form

Lyophilized from a 0.2 μm filtered solution in 20 mM NaH2PO4 and 500 mM NaCl, pH 7.0 with 50 μg BSA per 1 μg HGF

Preparation Note

Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. After reconstitution, this solution can be stored at 2-8 °C for up to one month or at -20 °C to -70 °C for up to three months. Us a manual defrost freezer and avoid repeated freeze-thaw cycles.

Analysis Note

The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osieosarcoma cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

Hepatocyte Growth Factor (HGF)

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.

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