MilliporeSigma
All Photos(1)

H1506

Sigma-Aldrich

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni

≥15 units/mg protein

Synonym(s):
3α-Hydroxysteroid:NAD(P)+ oxidoreductase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

bacterial (Pseudomonas testosteroni)

Quality Level

form

lyophilized solid

specific activity

≥15 units/mg protein

purified by

chromatography

composition

Protein, ≥25%

foreign activity

β-hydroxysteroid dehydrogenase ≤0.5%
alcohol dehydrogenase ≤0.05%

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Application

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosterone has been used:
  • in the preparation of composite electrode platform to for the determination of androsterone
  • in potassium phosphate buffer for the bioluminescent assay of bile acids in serum
  • for total bile salts assay using an enzymatic method

Packaging

50 units in glass bottle

Biochem/physiol Actions

3α-hydroxysteroid dehydrogenase (3α-HSD) may help to modulate the occupancy of the androgen receptor (AR) in androgen target tissues. It may also act in hindering androgen action.

Unit Definition

One unit will oxidize 1.0 μmole of androsterone per min at pH 8.9 at 25 °C in the presence of β-NAD+.

Physical form

Lyophilized powder containing potassium phosphate buffer salt, EDTA, and DL-dithiothreitol

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

SDS

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service