Product H17001 (Wnt3A, human) is a full-length protein.
Please find here the associated Uniprot entry:
https://www.uniprot.org/uniprotkb/P56704/entry#sequences
H17001
Wnt3A, human
recombinant, expressed in HEK 293 cells human
Synonym(s):
Wnt, Wnt-3A
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About This Item
NACRES:
NA.77
UNSPSC Code:
12352202
Form:
powder
Assay:
>90% (SDS-PAGE)
Recombinant:
expressed in HEK 293 cells human
Mol wt:
38-42 kDa
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recombinant
expressed in HEK 293 cells human
assay
>90% (SDS-PAGE)
form
powder
potency
≤20 ng/mL EC50
mol wt
38-42 kDa
impurities
<1 EU/μg endotoxin
storage temp.
−20°C
1 of 4
This Item | |||
|---|---|---|---|
| assay >90% (SDS-PAGE) | assay >98% (SDS-PAGE and HPLC) | assay ≥95% (SDS-PAGE) | assay - |
| recombinant expressed in HEK 293 cells human | recombinant - | recombinant expressed in HEK 293 cells | recombinant - |
| mol wt 38-42 kDa | mol wt - | mol wt dimer 26-30 kDa (glycosylated) | mol wt - |
| form powder | form - | form lyophilized powder | form buffered aqueous solution |
| storage temp. −20°C | storage temp. - | storage temp. −20°C | storage temp. −20°C |
| impurities <1 EU/μg endotoxin | impurities <0.1 μg/mg protein endotoxin (<1EU/μg) | impurities ≤1.00 EU/μg | impurities - |
General description
WNT3A (Wnt family member 3A) gene is mapped to human chromosome 1q42.13.[1] Wnt3A gene is a member of the Wnt gene family that consists of structurally linked genes that encode secreted signaling proteins.
Biochem/physiol Actions
WNT3A (Wnt family member 3A) functions mainly to induce canonical Wnt signaling pathway.[2] Wnt signaling pathway is essential for gastrulation, organogenesis, tissue development, homeostasis and regeneration. Congenital defects, oncogenic transformation and many more disorders results due to abnormal Wnt signaling pathway.[3] During the differentiation of human neuronal progenitor cells, WNT3A (Wnt family member 3A) activates calmodulin-dependent kinase II and increases free calcium in the cytosol via Wnt/Ca2+ signaling pathway.[4] WNT3A is an oncogene and is upregulated in many types of cancer. It serves as a prognostic marker in diagnosing hepatocellular carcinoma.[1][5] It also promotes metastasis of malignant tumors, including gastric cancer, colorectal cancer, non-small cell lung cancer and breast cancer.[6]
Physical form
Lyophilized from a 0.2 μm filtered solution of 1X PBS pH 7.4 + 500mM NaCl + 1% CHAPS.
Preparation Note
It is recommended to reconstitute the protein to ≤100μg/mL in sterile 1X PBS containing 0.1% endotoxin-free HSA.
Upon reconstitution, the cytokine can be stored at 4°C for short term or at -20°C to -80°C in aliquots for long term. Avoid repeated freeze-thaw cycles.
Analysis Note
The activity was determined by dose dependent Alkaline Phosphatase production in MC3T3 E1 mouse preosteoblast cells and stabilization of beta catenin in mouse L929 cells.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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The WNT target SP5 negatively regulates WNT transcriptional programs in human pluripotent stem cells
Huggins IJ, et al.
Nature Communications, 8(1), 1034-1034 (2017)
Ying Xu et al.
Frontiers in bioengineering and biotechnology, 8, 946-946 (2020-08-28)
Mesenchymal stromal cell senescence and apoptosis have been identified as critical molecular hallmarks in aging. In this study, we used stromal cell sheet culture as an in vitro model to study the progressive changes of cellular senescence, apoptosis and underlying
Expression of Wnt3a in hepatocellular carcinoma and its effects on cell cycle and metastasis
Lu C, et al.
International Journal of Oncology, 51(4), 1135-1145 (2017)
Abnormality of Wnt3a expression as novel specific biomarker for diagnosis and differentiation of hepatocellular carcinoma.
Pan L
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 37(4), 5561-5568 (2016)
The Wnt inhibitor LGK-974 enhances radiosensitivity of HepG2 cells by modulating Nrf2 signaling
Tian D, et al.
International Journal of Oncology, 51(2), 545-554 (2017)
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