H1759

(+)-Biotin N-hydroxysuccinimide ester

≥98% (HPLC), powder

• Synonym(s): (+)-Biotin N-succinimidyl ester, d-Biotin NHS ester, BNHS, Biotinyl-N-hydroxy-succinimide, N-Hydroxysuccinimidobiotin, N-Succinimidyl D-biotinate, NHS-Biotin
• Empirical Formula (Hill Notation): C₁₄H₁₉N₃O₅S
• CAS Number: 35013-72-0
• Molecular Weight: 341.38
• UNSPSC Code: 12352203
• NACRES: NA.46
• PubChem Substance ID: 24895479
• MDL number: MFCD00078531
• Beilstein/REAXYS Number: 4720781

Properties

• Quality Level: 200
• assay: ≥98% (HPLC)
• form: powder
• reaction suitability: reaction type: Biotinylations
• color: white to off-white
• mp: 212 °C
• solubility: DMF: ≤50 mg/mL (Stable for at least one month in dry DMF), DMSO: soluble
• storage temp.: −20°C
• SMILES string: [H][C@]12CS[C@@H](CCCCC(=O)ON3C(=O)CCC3=O)[C@@]1([H])NC(=O)N2
• InChI: 1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1
• InChI key: YMXHPSHLTSZXKH-RVBZMBCESA-N

Description

Application

(+) Biotin NHS ester or (+)-Biotin N-hydroxysuccinimide ester has been used:

• to biotinylate Hyb 246-4 for surfactant protein D enzyme-linked immunosorbent assay (SP-D ELISA)[1]
• to biotinylate proteins to form preformed complexes[2]
• to label and modify monoclonal anti-microfibrillar-associated protein 4 (MFAP4) (HG-HYB 7-18) to bind to alphaLISA streptavidin-coated donor beads[3]

Biochem/physiol Actions

The N-hydroxysuccinimide ester (NHS)-biotin modification helps in the biotinylation of the target lysine residue at its ε-amino group and identifies the surface lysine residues. It is used as a tool to identify the interaction of proteins with several biological molecules, like protein-polysaccharide interactions, protein-protein interactions, protein-nucleic acid interactions, and protein-ligand interactions.[4] NHS-biotin may be used in the biotinylation of proteins and peptides. It is typically coupled to primary amine in the pH range of 6.5-8.5.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)