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H2126

Sigma-Aldrich

Hyaluronidase from sheep testes

Type II, lyophilized powder, ≥300 units/mg solid

Synonym(s):
Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

conjugate

conjugate (Glucosaminoglycan)

Quality Level

type

Type II

form

lyophilized powder

specific activity

≥300 units/mg solid

mol wt

55 kDa

storage temp.

−20°C

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This Item
H6254H3631H3884
specific activity

≥300 units/mg solid

specific activity

≥1,500 units/mg solid

specific activity

3,000-15,000 units/mg solid

specific activity

750-3000 units/mg solid

mol wt

55 kDa

mol wt

55 kDa

mol wt

~55 kDa (four subunits of 14 kDa each)

mol wt

~55 kDa (four subunits of 14 kDa each)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

type

Type II

type

Type V

type

Type VI-S

type

Type IV-S

General description

Hyaluronidase is a hyaluronic acid-metabolizing enzyme. It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.

Application

Hyaluronidase from sheep testes has been used:
  • in flow cytometry
  • in the isolation of putative spermatogonial stem cells (SSCs)
  • to study the effects of magnetic stimulation in magnetic hydrogel properties
  • in immunohistochemistry to visualize collagen type II distribution
  • to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings
  • for the degeneration of hydrogels

Biochem/physiol Actions

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Physical form

Lyophilized powder containing lactose

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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25G
Pack Size/Quantity

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Side Effects of Drugs Annual: A world-wide yearly survey of new data and trends in adverse drug reactions., 603-619 (2010)
Multifunctional magnetic-responsive hydrogels to engineer tendon-to-bone interface
Silva ED, et al.
Nanomedicine: Nanotechnology, Biology, and Medicine, 14(7), 2375-2385 (2018)
Suppression of Sertoli cell tumour development during the first wave of spermatogenesis in inhibin $\alpha$-deficient mice
Haverfield JT, et al.
Reproduction, Fertility, and Development, 29(3), 609-620 (2017)
3D bioprinting of methacrylated hyaluronic acid (MeHA) hydrogel with intrinsic osteogenicity
Poldervaart MT, et al.
PLoS ONE, 12(6), e0177628-e0177628 (2017)
Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting
Mouser VHM, et al.
Biofabrication, 8(3), 035003-035003 (2016)

Articles

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

This procedure may be used for Hyaluronidase products.

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