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H2126

Sigma-Aldrich

Hyaluronidase from sheep testes

Type II, lyophilized powder, ≥300 units/mg solid

Synonym(s):

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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100 MG
$75.60
500 MG
$225.00
1 G
$383.00
5 G
$1,550.00

$75.60


Estimated to ship onApril 19, 2025



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100 MG
$75.60
500 MG
$225.00
1 G
$383.00
5 G
$1,550.00

About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

$75.60


Estimated to ship onApril 19, 2025


conjugate

conjugate (Glucosaminoglycan)

Quality Level

type

Type II

form

lyophilized powder

specific activity

≥300 units/mg solid

mol wt

55 kDa

storage temp.

−20°C

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General description

Hyaluronidase is a hyaluronic acid-metabolizing enzyme.[1] It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.[2]

Application

Hyaluronidase from sheep testes has been used:
  • in flow cytometry[3]
  • in the isolation of putative spermatogonial stem cells (SSCs)[4]
  • to study the effects of magnetic stimulation in magnetic hydrogel properties[5]
  • in immunohistochemistry to visualize collagen type II distribution[6][7]
  • to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings[8]
  • for the degeneration of hydrogels[9]

Biochem/physiol Actions

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Physical form

Lyophilized powder containing lactose

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Multifunctional magnetic-responsive hydrogels to engineer tendon-to-bone interface
Silva ED, et al.
Nanomedicine: Nanotechnology, Biology, and Medicine, 14(7), 2375-2385 (2018)
Suppression of Sertoli cell tumour development during the first wave of spermatogenesis in inhibin $\alpha$-deficient mice
Haverfield JT, et al.
Reproduction, Fertility, and Development, 29(3), 609-620 (2017)
Side Effects of Drugs Annual: A world-wide yearly survey of new data and trends in adverse drug reactions., 603-619 (2010)
Miscellaneous Drugs, Materials, Medical Devices and Techniques
Nan A
Side Effects of Drugs Annual, 37, 603-619 (2015)
Robert B Struijk et al.
PloS one, 15(3), e0230253-e0230253 (2020-03-17)
Autologous transplantation of spermatogonial stem cells is a promising new avenue to restore fertility in infertile recipients. Expansion of the initial spermatogonial stem cell pool through cell culturing is a necessary step to obtain enough cells for effective repopulation of

Articles

Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Protocols

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Questions

  1. H2126-100MG Hyaluronidase from sheep testes についてですが、溶解方法を教えてください。

    1 answer
    1. This enzyme is soluble at 1 mg/mL in 20 mM sodium phosphate buffer (pH 7.0), with 77 mM sodium chloride and 0.1 mg/mL BSA at 1 mg/mL. For assay purposes, this product is prepared at 0.3 mg/mL solution in Phosphate Buffer. Heating may be required, Please see the link below to review the full assay protocol:
      https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-hyaluronidase#preparation

      Please see the link below to review the product datasheet for a bovine Hyaluronidase, product H3884. This information may also be applied to the ovine source material:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/739/183/h3884pis-ms.pdf

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