This product may be reconstituted at 1 mg/mL in Tris-HCl, pH 7.5, containing 50 mM NaCl, 4 mM CaCl2, and 0.01% BSA. The solutio is stable for at least one week at -20 °C or 3 months at 70 °C. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/215/379/h2519pis-ms.pdf
H2519
Heparinase I from Flavobacterium heparinum
Lyophilized powder stabilized with approx. 25% bovine serum albumin, ≥200 units/mg protein (enzyme + BSA)
Synonym(s):
Heparin lyase I, Heparinase
Sign In to View Organizational & Contract Pricing.
Select a Size
50 UNITS
$410.00
100 UNITS
$737.00
250 UNITS
$1,570.00
$410.00
Check Cart for Availability
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Skip To
Product Name
Heparinase I from Flavobacterium heparinum, Lyophilized powder stabilized with approx. 25% bovine serum albumin, ≥200 units/mg protein (enzyme + BSA)
conjugate
conjugate (Glucosaminoglycan)
form
lyophilized solid
specific activity
≥200 units/mg protein (enzyme + BSA)
mol wt
42.8 kDa
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
Quality Level
Looking for similar products? Visit Product Comparison Guide
1 of 4
This Item | H8891 | H6512 | H3917 |
|---|---|---|---|
| specific activity ≥200 units/mg protein (enzyme + BSA) | specific activity ≥30 units/mg protein (enzyme + BSA) | specific activity ≥100 units/mg protein (enzyme + BSA) | specific activity ≥200 units/mg protein |
| form lyophilized solid | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| mol wt 42.8 kDa | mol wt 70.8 kDa | mol wt 84.1 kDa | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Application
Biochem/physiol Actions
Other Notes
One unit (Sigma unit) will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Methodological aspects affecting the infectivity of Chlamydia pneumoniae in cell cultures in vitro
Yan Y, et al.
Journal of Microbiological Methods, 61(1), 127-130 (2005)
Wataru Hashimoto et al.
Biochemistry, 53(4), 777-786 (2014-01-21)
Pedobacter heparinus (formerly known as Flavobacterium heparinum) is a typical glycosaminoglycan-degrading bacterium that produces three heparin lyases, Hep I, Hep II, and Hep III, which act on heparins with 1,4-glycoside bonds between uronate and amino sugar residues. Being different from
HeparinHeparin - A Century of Progress, 273-273 (2012)
Cardiopulmonary Bypass: Principles and Practice, 509-509 (2008)
Avery Davis Bell et al.
Nature, 583(7815), 259-264 (2020-06-05)
Meiosis, although essential for reproduction, is also variable and error-prone: rates of chromosome crossover vary among gametes, between the sexes, and among humans of the same sex, and chromosome missegregation leads to abnormal chromosome numbers (aneuploidy)1-8. To study diverse meiotic
Articles
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
-
How to reconstitute and how to save after reconstituting?
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service