MilliporeSigma
All Photos(5)

H3506

Sigma-Aldrich

Hyaluronidase from bovine testes

Type I-S, lyophilized powder, 400-1000 units/mg solid

Synonym(s):
Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

type

Type I-S

Quality Level

form

lyophilized powder

specific activity

400-1000 units/mg solid

mol wt

~55 kDa (four subunits of 14 kDa each)

technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

UniProt accession no.

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

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This Item
H3884H3631H3757
form

lyophilized powder

form

lyophilized powder (essentially salt-free)

form

lyophilized powder

form

lyophilized powder

specific activity

400-1000 units/mg solid

specific activity

750-3000 units/mg solid

specific activity

3,000-15,000 units/mg solid

specific activity

300-1,000 U/mg

mol wt

~55 kDa (four subunits of 14 kDa each)

mol wt

~55 kDa (four subunits of 14 kDa each)

mol wt

~55 kDa (four subunits of 14 kDa each)

mol wt

~55 kDa (four subunits of 14 kDa each)

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

-

technique(s)

-

technique(s)

-

UniProt accession no.

Q5E985, Q8SQG8

UniProt accession no.

Q5E985, Q8SQG8

UniProt accession no.

Q5E985, Q8SQG8

UniProt accession no.

Q5E985, Q8SQG8

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

Application

Hyaluronidase from bovine testes has been used for the digestion of breast tissue, heart tissue and oocytes for isolation of cells.

Suitable for use in preparation of single cell suspension for sequencing.

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.
These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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