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MilliporeSigma

H3662

Sigma-Aldrich

HEPES sodium salt solution

1M, BioReagent, suitable for cell culture

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About This Item

Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
75277-39-3
Molecular Weight:
260.29
MDL number:
MFCD00036463
UNSPSC Code:
12161700
PubChem Substance ID:
24895590
NACRES:
NA.25

Key Documents

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Quality Level

200

sterility

sterile-filtered (0.2 μm filtered)

product line

BioReagent

form

solution

concentration

0.90-1.10 M

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested
 DNase, RNase. Protease, Nickase, free
 endotoxin, tested
<5 ppm Heavy metals (as Pb)

cation traces

Fe: <5 ppm

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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This Item
RDD035391333H7006
HEPES sodium salt solution 1M, BioReagent, suitable for cell culture

Sigma-Aldrich

H3662

HEPES sodium salt solution

HEPES sodium salt &#8805;99.5% (titration), free-flowing, Redi-Dri&#8482;

Sigma-Aldrich

RDD035

HEPES sodium salt

HEPES sodium salt ULTROL&#174; Grade, &#8805;99% dry basis (titration), zwitterionic N-substituted aminosulfonic acid buffer

Millipore

391333

HEPES sodium salt

HEPES sodium salt &#8805;99.0% (titration)

Sigma-Aldrich

H7006

HEPES sodium salt

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

HPLC: suitable

concentration

0.90-1.10 M

concentration

-

concentration

-

concentration

-

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

400

product line

BioReagent

product line

Redi-Dri™

product line

-

product line

-

form

solution

form

-

form

solid

form

powder

impurities

Bioburden, tested, endotoxin, tested, DNase, RNase. Protease, Nickase, free, <5 ppm Heavy metals (as Pb)

impurities

-

impurities

-

impurities

-

General description

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966.[1] It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.

Application

HEPES sodium salt solution is suitable for use in cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during phosphorylation assays in permeabilized fibroblasts.[2] It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.[3]

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000431723
0000431722
0000431724
0000431722
0000431723

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Hydrogen ion buffers for biological research.
Good NE, et al.
BioChemistry: An Indian Journal, 5, 467-477 (1996)
I J Chopra et al.
Thyroid : official journal of the American Thyroid Association, 6(4), 255-259 (1996-08-01)
We have devised a practical, sensitive, and reliable assay for measurement of free T3 concentration in serum. The assay employs a convenient and disposable plastic equilibrium dialysis cell and a buffer that resembles the in vivo biochemical environment (Nelson JC
C Estrada et al.
The Biochemical journal, 326 ( Pt 2), 369-376 (1997-09-18)
Although it has been demonstrated that NO inhibits the proliferation of different cell types, the mechanisms of its anti-mitotic action are not well understood. In this work we have studied the possible interaction of NO with the epidermal growth factor
Jihye Park et al.
The Analyst, 137(19), 4411-4414 (2012-08-07)
We have developed a quickly responsive, and specific fluorescent assay for the detection of Au(III) on the basis of the formation of gold nanoparticles in the presence of HEPES, which cleave the C-I bond of I-BODIPY 1 to yield the
S Hugel et al.
British journal of pharmacology, 166(8), 2402-2416 (2012-03-29)
Artificial buffers such as HEPES are extensively used to control extracellular pH (pH(e) ) to investigate the effect of H(+) ions on GABA(A) receptor function. In neurones cultured from spinal cord dorsal horn (DH), dorsal root ganglia (DRG) and cerebellar
View All Related Papers

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