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H3662

Sigma-Aldrich

HEPES sodium salt solution

BioReagent, 1M, suitable for cell culture

Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
Molecular Weight:
260.29
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

sterility

sterile-filtered (0.2 μm filtered)

product line

BioReagent

form

solution

concentration

0.90-1.10 M

application(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested
DNase, RNase. Protease, Nickase, free
endotoxin, tested
<5 ppm Heavy metals (as Pb)

cation traces

Fe: <5 ppm

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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General description

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.

Application

HEPES sodium salt solution is suitable for use in cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

C Estrada et al.
The Biochemical journal, 326 ( Pt 2), 369-376 (1997-09-18)
Although it has been demonstrated that NO inhibits the proliferation of different cell types, the mechanisms of its anti-mitotic action are not well understood. In this work we have studied the possible interaction of NO with the epidermal growth factor...
I J Chopra et al.
Thyroid : official journal of the American Thyroid Association, 6(4), 255-259 (1996-08-01)
We have devised a practical, sensitive, and reliable assay for measurement of free T3 concentration in serum. The assay employs a convenient and disposable plastic equilibrium dialysis cell and a buffer that resembles the in vivo biochemical environment (Nelson JC...
Hydrogen ion buffers for biological research.
Good NE, et al.
BioChemistry: An Indian Journal, 5, 467-477 (1996)
Peng Yang
Macromolecular bioscience, 12(8), 1053-1059 (2012-06-19)
When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue"...
Rakesh K Pathak et al.
Analytical chemistry, 84(11), 5117-5123 (2012-05-04)
An in situ prepared Zn(2+) complex of triazole linked imino-thiophenyl conjugate of calix[4]arene, [ZnL], was demonstrated to be highly fluorescent in HEPES buffer solution. [ZnL] has been used as a chemo-sensing ensemble for the recognition of phosphates in general and...

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