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H3784

Sigma-Aldrich

HEPES sodium salt

BioPerformance Certified, suitable for cell culture, ≥99.0%

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Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
Molecular Weight:
260.29
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

BioPerformance Certified

Quality Level

assay

≥99.0%

form

powder

storage condition

dry at room temperature

technique(s)

activity assay: suitable
cell culture | mammalian: suitable

impurities

endotoxin and total aerobic microbial count, tested

color

white

pH

10-12 (1 M in water)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 0.1 M, clear, colorless

density

1.19 g/cm3 at 20—25 °C
1.21 g/cm3 at 20—25 °C

absorption

≤0.02 at 280 in H2O at 0.1 M
≤0.04 at 260 in H2O at 0.1 M

suitability

suitable for component for culture media
suitable for molecular biology

application(s)

cell analysis
diagnostic assay manufacturing
general analytical
life science and biopharma

foreign activity

DNase, RNase, NICKase, protease, none detected

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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HEPES sodium salt BioPerformance Certified, suitable for cell culture, ≥99.0%

H3784

HEPES sodium salt

Premium Grade
HEPES sodium salt ≥99.0% (titration)

H7006

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-
HEPES sodium salt solution 1M, BioReagent, suitable for cell culture

H3662

HEPES sodium salt solution

Essential+ Grade
HEPES sodium salt ≥99.5% (titration), free-flowing, Redi-Dri™

RDD035

HEPES sodium salt

-
grade

BioPerformance Certified

grade

-

grade

-

grade

-

technique(s)

activity assay: suitable, cell culture | mammalian: suitable

technique(s)

HPLC: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

Quality Level

400

Quality Level

400

Quality Level

200

Quality Level

100

application(s)

cell analysis
diagnostic assay manufacturing
general analytical
life science and biopharma

application(s)

diagnostic assay manufacturing
general analytical (
)
life science and biopharma
metabolomics

application(s)

-

application(s)

diagnostic assay manufacturing

form

powder

form

powder

form

solution

form

-

General description

HEPES, or 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, is a zwitterionic N-substituted aminosulfonic acid buffer with a remarkable pH buffering capacity between 6.8 and 8.2 (pKa of 7.48 at 25°C). Widely recognized for its versatility, HEPES is extensively employed in cell biology, biochemical, and biological research. Notably, its neutrality towards metal ions makes it a preferred choice for solutions with metal ions, acting as a "non-coordinating buffer."

In cell culture, HEPES excels in maintaining stable physiological pH even amidst fluctuating carbon dioxide concentrations, outperforming bicarbonate buffers (NaHCO3). Moreover, HEPES showcases valuable properties in various biological and biochemical processes, such as isoelectric focusing for protein separation and minimal interference in DNA-restriction enzyme reactions, making it indispensable across diverse research applications, including immunoprecipitation, cell lysis, and live cell imaging. Its versatility, exceptional buffering capacity, and minimal interaction with molecules establish HEPES as a key tool in various research domains.

Application

HEPES sodium salt has been used:
  • to supplement the HSOF medium
  • as a buffer component of HCZB medium in egg and embryo culture
  • as a buffer component in cell culture

Features and Benefits

  • High-purity product for biochemical and biological research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C
  • Suitable for Cell culture
  • Tested for Endotoxins and Total Aerobic Microbial Count
  • Free from DNase, NICKase, RNase, Endonuclease, Exonuclease and Protease

Physical form

Lyophilized, spray dried, or crystallized material.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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