HEPES sodium salt

BioPerformance Certified, suitable for cell culture, ≥99.5%

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:


BioPerformance Certified

Quality Level






cell culture | mammalian: suitable


endotoxin and total aerobic microbial count, tested


10-12 (1 M in water)

useful pH range

6.8 - 8.2

pKa (25 °C)



water: 0.1 M, clear, colorless (260 mg/mL)


≤0.02 at 280 in H2O at 0.1 M
≤0.04 at 260 in H2O at 0.1 M

Featured Industry

Diagnostic Assay Manufacturing

foreign activity

DNase, RNase, NICKase, protease, none detected

SMILES string




InChI key


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25, 100, 500 g in poly bottle
1 kg in poly bottle

Physical form

Lyophilized, spray dried, or crystallized material.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Ekaterina Isaac et al.
Theriogenology, 161, 273-284 (2020-12-29)
The growth of viable cattle embryos in culture to stages beyond the hatching blastocyst is of interest to developmental biologists wishing to understand developmental events beyond the first lineage decision, as well as for commercial applications, because a lengthening of...
Ines Rosignol et al.
International journal of molecular sciences, 21(5) (2020-03-14)
Mitochondrial damage plays a prominent role in glaucoma. The only way cells can degrade whole mitochondria is via autophagy, in a process called mitophagy. Thus, studying mitophagy in the context of glaucoma is essential to understand the disease. Up to...
Mingyue Li et al.
Structure (London, England : 1993), 27(5), 806-815 (2019-03-19)
The peroxidation of cardiolipins by reactive oxygen species, which is regulated and enhanced by cytochrome c (cyt c), is a critical signaling event in mitochondrial apoptosis. We probe the molecular underpinnings of this mitochondrial death signal through structural and functional...
Artem Babaian et al.
Cell reports, 31(5), 107611-107611 (2020-05-07)
The ribosome is an RNA-protein complex that is essential for translation in all domains of life. The structural and catalytic core of the ribosome is its ribosomal RNA (rRNA). While mutations in ribosomal protein (RP) genes are known drivers of...
S Hugel et al.
British journal of pharmacology, 166(8), 2402-2416 (2012-03-29)
Artificial buffers such as HEPES are extensively used to control extracellular pH (pH(e) ) to investigate the effect of H(+) ions on GABA(A) receptor function. In neurones cultured from spinal cord dorsal horn (DH), dorsal root ganglia (DRG) and cerebellar...

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