H3884
bovine testis
Type IV-S
lyophilized powder (essentially salt-free)
750-3000 units/mg solid
~55 kDa (four subunits of 14 kDa each)
diagnostic assay manufacturing
cancer
−20°C
cow ... HYAL1(515397) , HYAL2(281838)
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11 - Combustible Solids
WGK 3
Not applicable
Not applicable
dust mask type N95 (US), Eyeshields, Gloves
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.
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Although Sigma-Aldrich has not tested the stability of stock solutions of Product H3884, Hyaluronidase from bovine testes, several publications cite storage of solutions of H3884 at -20 °C:1. Campbell, Keith H.S, "Transgenic Sheep form Cultured Cells", from Transgenesis Techniques: Principles and Protocols, Methods Mol. Biol., 180, 289-301 (2002). A 1500 IU/mL stock solution was stored in 20 mL aliquots at -20 °C.2. Marschall, S. and de Angelis, M.H., "In Vitro Fertilization/Cryopreservation", from Transgenic Mouse: Methods and Protocols, Methods in Molecular Biology, 209, pp. 35-50. Humana Press (2003). 1 mg/mL stock solutions in M2 medium or water, sterile-filtered (0.22 mm filter) can be stored in aliquots at -20 °C.
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A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
This procedure may be used for Hyaluronidase products.
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