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H3884

Sigma-Aldrich

Hyaluronidase from bovine testes

Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid

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Synonym(s):
Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
CAS Number:
37326-33-3
Enzyme Commission number:
3.2.1.35 (BRENDA, IUBMB)
EC Number:
253-464-3
MDL number:
MFCD00131351

biological source

bovine testis

Quality Level

200

conjugate

conjugate (Glucosaminoglycan)

type

Type IV-S

form

lyophilized powder (essentially salt-free)

specific activity

750-3000 units/mg solid

mol wt

~55 kDa (four subunits of 14 kDa each)

UniProt accession no.

Q5E985
Q8SQG8

application(s)

diagnostic assay manufacturing

relevant disease(s)

cancer

storage temp.

−20°C

Gene Information

cow ... HYAL1(515397) , HYAL2(281838)

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This Item
H3631H3506H4272
Hyaluronidase from bovine testes Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid

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H3884

Hyaluronidase from bovine testes

Hyaluronidase from bovine testes Type VI-S, lyophilized powder, 3,000-15,000 units/mg solid

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H3631

Hyaluronidase from bovine testes

Hyaluronidase from bovine testes Type I-S, lyophilized powder, 400-1000 units/mg solid

Sigma-Aldrich

H3506

Hyaluronidase from bovine testes

Hyaluronidase from bovine testes Type IV-S, powder, suitable for mouse embryo cell culture, 750-3000 units/mg solid

Sigma-Aldrich

H4272

Hyaluronidase from bovine testes

form

lyophilized powder (essentially salt-free)

form

lyophilized powder

form

lyophilized powder

form

powder

specific activity

750-3000 units/mg solid

specific activity

3,000-15,000 units/mg solid

specific activity

400-1000 units/mg solid

specific activity

750-3000 units/mg solid

mol wt

~55 kDa (four subunits of 14 kDa each)

mol wt

~55 kDa (four subunits of 14 kDa each)

mol wt

~55 kDa (four subunits of 14 kDa each)

mol wt

~55 kDa (four subunits of 14 kDa each)

UniProt accession no.

Q5E985, Q8SQG8

UniProt accession no.

Q5E985, Q8SQG8

UniProt accession no.

Q5E985, Q8SQG8

UniProt accession no.

-

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing

application(s)

-

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.
These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

This procedure may be used for Hyaluronidase products.

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