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H4149

Sigma-Aldrich

Monoclonal Anti-Heat Shock Protein 60 antibody produced in mouse

clone LK1, ascites fluid

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MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

LK1, monoclonal

mol wt

antigen 60 kDa

contains

15 mM sodium azide

species reactivity

rat, human, chicken

technique(s)

electron microscopy: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: suitable
western blot: 1:400 using cultured human foreskin fibroblast extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HSPD1(3329)
rat ... Hspd1(63868)

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H3524H1775H5147
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

species reactivity

rat, human, chicken

species reactivity

spinach, chicken, E. coli, helminth, rat, human

species reactivity

wheat germ, chicken, Sf9 cell line, rabbit, rat, human, mouse, Achlya

species reactivity

bovine, chicken, guinea pig, plant, Drosophila, rat, human, nematode, rabbit, hamster

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

LK1, monoclonal

clone

LK2, monoclonal

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AC-16, monoclonal

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BRM-22, monoclonal

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General description

Heat shock (stress) proteins (HSP) are produced by prokaryotic and eukaryotic cells, and are among the most conserved molecules in phylogeny. HSP-60 is a member of HSP family with a uniquely high level of sequence conservation.
Monoclonal Anti-Heat Shock Protein 60 (mouse IgG1 isotype) is derived from the LK1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human heat diseases.

Specificity

The antibody recognizes an epitope located between amino acid residues 383-447 of the human HSP 60. It is not cross-reactive with the bacterial counterpart (E. coli), or with helminth and spinach HSP60. The antibody reacts with constitutive and inducible HSP60. It demonstrates a raised level of staining in immunohistochemistry of synovial membranes taken from patients with juvenile chronic arthritis.

Immunogen

recombinant human heat shock protein 60 (HSP60)

Application

Monoclonal Anti-Heat Shock Protein 60 has been used in the following techniques:
  • Enzyme linked immunosorbent assay (ELISA)
  • Immunoblotting
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoelectron microscopy
  • Double immunofluorescence

Biochem/physiol Actions

A wide variety of environmental perturbations, such as a sudden increase in temperature, induce cells to rapidly synthesize a group of polypeptides known as the heat shock (stress) proteins. HSP-60 is considered to be a potential antigen in a number of autoimmune diseases. Abnormal immune reactivity involving shock protein 60 (HSP60) has also been implicated in the pathogenesis of schizophrenia. This monoclonal antibody enables the differentiation between the HSP60 of mammalian and bacterial origin.
Abnormal immune reactivity involving HSP60 has also been implicated in the pathogenesis of schizophrenia.

Physical form

Monoclonal Anti-Heat Shock Protein 60 is provided as ascites fluid with 0.1% sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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P Costantini et al.
Cell death and differentiation, 9(1), 82-88 (2002-01-23)
As shown here, mitochondria purified from different organs (liver, brain, kidney, spleen and heart) contain both pro-caspase-9 and the processed, mature form of caspase-9. Purified liver mitochondria release mature caspase-9 upon induction of permeability transition in vitro. This is accompanied
Studies characterizing 60 kda autoantibodies in subjects with schizophrenia
Wang X, et al.
Biological Psychiatry, 53(5), 361-375 (2003)
HSP60: issues and insights on its therapeutic use as an immunoregulatory agent
Coelho V and Faria A
Frontiers in Immunology, 2, 97-97 (2012)
Claudia Campanella et al.
Oncotarget, 7(20), 28849-28867 (2015-12-25)
HSP60 undergoes changes in quantity and distribution in some types of tumors suggesting a participation of the chaperonin in the mechanism of transformation and cancer progression. Suberoylanilide hydroxamic acid (SAHA), a member of a family of histone deacetylase inhibitors (HDACi)
Antonella Marino Gammazza et al.
Cell stress & chaperones, 19(3), 343-353 (2013-09-24)
The role Hsp60 might play in various inflammatory and autoimmune diseases is under investigation, but little information exists pertaining to Hashimoto's thyroiditis (HT). With the aim to fill this gap, in the present work, we directed our attention to Hsp60

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