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Key Documents

H4652

Sigma-Aldrich

HMG-1 Peptide

≥90% (SDS-PAGE), lyophilized powder, Histidine-tagged, human recombinant, expressed in E. coli

Synonym(s):

Amphoterin, HMGB1, High-Mobility-Group protein

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About This Item

MDL number:
UNSPSC Code:
12352202
PubChem Substance ID:
NACRES:
NA.42

product name

HMG-1 human, lyophilized powder, ≥90% (SDS-PAGE), Histidine-tagged, recombinant, expressed in E. coli

recombinant

expressed in E. coli

Quality Level

assay

≥90% (SDS-PAGE)

form

lyophilized powder

mol wt

25 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

SMILES string

COC(=O)CCCCCCCCCCCCCCO

Gene Information

human ... HMGB1(3146)

General description

215 amino acids; comprises two homologous HMG boxes, rich in basic amino acids, and an acidic tail at the carboxy-terminus
HMG-1 (high mobility group protein) is a nuclear protein of molecular weight 30kDa. It is secreted from macrophages, monocytes and necrotic cells. It is ubiquitously expressed. The protein has basic HMG (high mobility group) box domains and an acidic carboxyl-terminus.

Application

HMG-1 (high-mobility-group protein) human has been used:
  • to prepare a standard curve for measuring HMG1 using ELISA (enzyme-linked immunosorbent assay) test
  • to study the apoptotic ratios of Lewis cells in presence of exogenous HMG-1
  • to study the changes in Th (T helper)-17 cell expansion in the presence of HMG-1 under in vitro conditions
  • for the migration assay of GM7373 cells

Biochem/physiol Actions

HMG-1 (high mobility group protein) is a non-histone chromosomal protein, which promotes the sequence specific DNA attachment of proteins. HMG1 binds DNA in a non-sequence specific manner. It is involved in DNA replication, transcription and repair. In addition, it is up-regulated in cancers and is associated with cellular growth, vasculogenesis and apoptosis regulation. In the presence of infection or injury, HMG1 is secreted and mediates inflammation. It is associated with the pathogenesis of autoimmune diseases, such as rheumatoid arthritis (RA), inflammatory bowel disease (IBD) and systemic lupus erythematosus (SLE).

Physical form

Lyophilized powder containing HEPES buffer salts, pH 7.8, NaCl, EDTA, Triton X-100, Leupeptin and AEBSF.

Analysis Note

Tested in DNA retardation assay

pictograms

CorrosionEnvironment

signalword

Danger

hcodes

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


Certificates of Analysis (COA)

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H Wang et al.
Science (New York, N.Y.), 285(5425), 248-251 (1999-07-10)
Endotoxin, a constituent of Gram-negative bacteria, stimulates macrophages to release large quantities of tumor necrosis factor (TNF) and interleukin-1 (IL-1), which can precipitate tissue injury and lethal shock (endotoxemia). Antagonists of TNF and IL-1 have shown limited efficacy in clinical
Julia Matena et al.
International journal of molecular sciences, 16(4), 7478-7492 (2015-04-08)
To improve well-known titanium implants, pores can be used for increasing bone formation and close bone-implant interface. Selective Laser Melting (SLM) enables the production of any geometry and was used for implant production with 250-µm pore size. The used pore
X Xu et al.
Scandinavian journal of immunology, 79(6), 386-394 (2014-03-29)
Upregulated high-mobility group box 1 (HMGB1) has been found in many diseases. Nevertheless, the function of HMGB1 on modulating the proliferation of lung cancer cells (Lewis cells) and inhibiting apoptosis is poorly understood, as well as the involved intracellular signalling.
Tian-Tian Ma et al.
PloS one, 10(4), e0123586-e0123586 (2015-04-18)
High mobility group box-1 (HMGB1), a kind of pro-inflammatory mediator, is associated with inflammatory conditions and tissue damage. Our previous study demonstrated that the circulating levels of HMGB1 correlated with disease activity of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). In
A Taguchi et al.
Nature, 405(6784), 354-360 (2000-06-01)
The receptor for advanced glycation end products (RAGE), a multi-ligand member of the immunoglobulin superfamily of cell surface molecules, interacts with distinct molecules implicated in homeostasis, development and inflammation, and certain diseases such as diabetes and Alzheimer's disease. Engagement of

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