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H4791

Sigma-Aldrich

Bone Morphogenetic Protein 2 human

BMP-2, recombinant, expressed in HEK 293 cells, HumanKine, suitable for cell culture

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Synonym(s):
BMP-2
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in HEK 293 cells

form

lyophilized powder

potency

≤60 ng/mL EC50

quality

endotoxin tested

mol wt

dimer 30-38 kDa (glycosylated)

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg Endotoxin level

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... BMP2(650)

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This Item
B3555B1814B2805
recombinant

expressed in HEK 293 cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in NSO cells

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

potency

≤60 ng/mL EC50

potency

-

potency

-

potency

0.05-0.15 μg/mL ED50

mol wt

dimer 30-38 kDa (glycosylated)

mol wt

26 kDa

mol wt

26 kDa

mol wt

30–38 kDa

packaging

pkg of 10 μg

packaging

pkg of 10 μg

packaging

pkg of 10 μg

packaging

pkg of 20 μg

General description

Bone Morphogenetic Protein 2 is a member of the TGF-β superfamily of cytokines that affect bone and cartilage formation. It is important for skeletal development during embryogenesis. BMP-2 induces chondrocyte formation, osteoblast differentiation, and is involved in embryo dorsal-ventral patterning and organogenesis.

Biochem/physiol Actions

It has been reported that Bone Morphogenetic Protein 2 (BMP-2) inhibits estradiol induced proliferation of human breast cancer cells. BMP-2 signaling mediates apoptosis by activation of the TAK1-p38 kinase pathway that is negatively regulated by Smad6. Cellular responses to BMP-2 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors, which play significant roles in BMP binding and signaling. One BMP type II receptor and two BMP type I receptors have been identified. Both BMP type I receptors bind BMP-2 with high-affinity in the absence of BMP receptor type II.

Physical form

Lyophilized from a 0.2 μm filtered solution of 2x PBS + 6% Ethanol.

Preparation Note

This Bone Morphogenetic Protein 2 (BMP-2) is produced from a DNA sequence encoding the human BMP-2 protein, expressed in HEK 293 cells. It is a glycosylated homodimer linked by a single disulfide bond with an apparent molecular mass of 30-38 kDa.

Analysis Note

The specific activity was determined by its ability to induce alkaline phosphatase production in a dose response to BMP-2 in the ATDC-5 cell line (mouse chondrogenic cell line).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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