H6041

Interferon α 2A human

HumanKine^®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyopholized powder, suitable for cell culture

• Synonym(s): α 2A Interferon, Human Interferon α 2A, IFN-α 2A
• UNSPSC Code: 12352202
• NACRES: NA.77
• Biological source: human
• Recombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: monomer 16 kDa (glycosylated)
• Impurities: ≤1EU/mg

Properties

• Product Name: Interferon α 2A human, IFN-α 2A, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• biological source: human
• recombinant: expressed in HEK 293 cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤0.4 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: monomer 16 kDa (glycosylated)
• packaging: glass bottle of 10 μg (in glass bottle)
• manufacturer/tradename: HumanZyme
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1EU/mg
• UniProt accession no.: P01563
• storage temp.: −20°C
• Quality Level: 100
• Gene Information: human ... IFNA2(3440)

Description

Analysis Note

The activity is determined by the dose-dependent cytotoxicity of the human TF-1 cell line (human erythroleukemic indicator cell line)) ≤ 0.4.

Application

Interferon α 2A human has been used to check its antiviral action against chikungunya virus.[1] It has also been used as a control to check the antiviral cytokine induction of Korean red ginseng.[2]

Biochem/physiol Actions

IFNA2 is a type 1 interferon which acts as the first line of defense, and offers protection against pathogenic invasion.[3]

Interferon α 2A (IFN-α 2A) belongs to the type I interferon protein family and is produced by many cell types in response to viral infection. It has antiproliferative and antitumor activities, as well as induction of apoptosis and the inflammatory response. IFN-α 2A enhances the expression of MHC proteins by increasing the presentation of antigen peptides and activates cytotoxic T cells.

General description

The IFNA2 (interferon alpha 2) gene is mapped to human chromosome 9p21.3.[4] It is considered to be a prototypic type I IFN subtype. The encoded protein contains 165 amino acid. Ifna2 protein structure possesses five α-helices, linked by two disulfide bonds.[5]

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Preparation Note

HumanKine IFN-α 2A, expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 16 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable