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H6533
Anti-HA−Peroxidase antibody, Mouse monoclonal
clone HA-7, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin
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1 VIAL
$969.00
$969.00
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About This Item
Conjugate:
peroxidase conjugate
Clone:
HA-7, monoclonal
Application:
WB
Citations:
135
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biological source
mouse
conjugate
peroxidase conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HA-7, monoclonal
form
lyophilized powder
packaging
vial of 0.5 mL
concentration
5-11 mg/mL
technique(s)
western blot: 1:4,000 using using HA tagged fusion protein expressed in bacteria, and ECL immunoblotting detection reagent
storage temp.
2-8°C
Quality Level
1 of 4
This Item | |||
|---|---|---|---|
| clone HA-7, monoclonal | clone polyclonal | clone HIS-1, monoclonal | clone HA-7, monoclonal |
| conjugate peroxidase conjugate | conjugate unconjugated | conjugate peroxidase conjugate | conjugate unconjugated |
| antibody form purified from hybridoma cell culture | antibody form affinity isolated antibody | antibody form purified from hybridoma cell culture | antibody form ascites fluid |
| biological source mouse | biological source rabbit | biological source mouse | biological source mouse |
| form lyophilized powder | form buffered aqueous solution | form lyophilized powder | form - |
| technique(s) western blot: 1:4,000 using using HA tagged fusion protein expressed in bacteria, and ECL immunoblotting detection reagent | technique(s) immunoprecipitation (IP): 2.5-4 μg/test using HA-tagged fusion protein from cell lysates, indirect immunofluorescence: 10-20 μg/mL using HA-tagged fusion protein transfected cells, western blot: 0.5-0.8 μg/mL using HA-tagged fusion protein transfected cell extracts | technique(s) western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein | technique(s) immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein |
General description
Influenza A virus, a RNA virus, encodes two surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA). HA stalk domain based epitope region antibody is effective in neutralizing divergent influenza virus strains.
The mouse monoclonal Anti-HA-Peroxidase antibody recognizes native and denatured-reduced forms of the HA-tagged proteins. It also reacts with the N- or C-terminal HA-tagged fusion proteins expressed in E. coli and mammalian cells.
Immunogen
synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH
Application
Monoclonal Anti-HA-Peroxidase antibody produced in mouse has been used:
- in immunopreciptation and immunostaining of human member 9 of solute carrier family 38 SLC38A9 transfected in HeLa cells[1]
- in immunoblot detection in transfected A375 cells
- is suitable for immunoblotting of nucleosome assembly protein NAP1L1
Biochem/physiol Actions
The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.
Physical form
Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.
Preparation Note
Reconstitute in .5mL of distilled water.
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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A broadly reactive human anti-hemagglutinin stem monoclonal antibody that inhibits influenza a virus particle release
Yamayoshi S, et al.
EBioMedicine, 17, 182-191 (2017)
Influenza virus hemagglutinin stalk-based antibodies and vaccines
Krammer F and Palese P
Current Opinion in Virology, 3(5), 521-530 (2013)
Ernest C So et al.
The Journal of biological chemistry, 291(11), 5832-5843 (2016-01-13)
Legionella pneumophila, the causative agent of Legionnaire's disease, uses its type IV secretion system to translocate over 300 effector proteins into host cells. These effectors subvert host cell signaling pathways to ensure bacterial proliferation. Despite their importance for pathogenesis, the
Paulina Bartuzi et al.
Nature communications, 7, 10961-10961 (2016-03-12)
The low-density lipoprotein receptor (LDLR) plays a pivotal role in clearing atherogenic circulating low-density lipoprotein (LDL) cholesterol. Here we show that the COMMD/CCDC22/CCDC93 (CCC) and the Wiskott-Aldrich syndrome protein and SCAR homologue (WASH) complexes are both crucial for endosomal sorting
Joanna F McGouran et al.
Chemistry & biology, 20(12), 1447-1455 (2013-12-03)
Posttranslational modification with ubiquitin (Ub) controls many cellular processes, and aberrant ubiquitination can contribute to cancer, immunopathology, and neurodegeneration. The versatility arises from the ability of Ub to form polymer chains with eight distinct linkages via lysine side chains and
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What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?
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Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.
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