H6541

Oncostatin M human

OSM, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture

• Synonym(s): OSM
• CAS Number: 106956-32-5
• MDL number: MFCD00166731
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in HEK 293 cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤0.1-1.5 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: dimer 30 kDa (glycosylated)
• packaging: pkg of 5X10 μg; pkg of 10 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg
• UniProt accession no.: P13725
• storage temp.: −20°C
• Gene Information: human ... OSM(5008)

Description

Biochem/physiol Actions

Oncostatin M (OSM), LIF, G-CSF, IL-6, and CNTF are structurally related members of the same cytokine family sharing similarities in their primary amino acid sequences, predicted secondary structure, and receptor components. OSM is a growth-regulating cytokine, affecting a number of tumor and normal cells. This material was first identified by its ability to inhibit the growth of A375 melanoma cells and other human tumor cells, but not inhibit the growth of normal human fibroblasts. It acts synergistically with TGF β1 to inhibit the proliferation of tumor cells like A375 melanoma cells. It induces an increase in LDL receptor expression and LDL uptake by hepatoma cells. OSM activates synovial fibroblast-like cells to produce urokinase type plasminogen activator. OSM is secreted by macrophages and activated T lymphocytes.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Analysis Note

The activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (humanerythroleukemic indicator cell line)

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable