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H6648

Sigma-Aldrich

Hanks′ Balanced Salt solution

Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

HBSS

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

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Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: no

shipped in

ambient

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This Item
55021CH9394H8264
Sigma-Aldrich

Sigma-Aldrich

H6648

Hanks′ Balanced Salt solution

Hanks’ Balanced Salt solution HBSS Modified, with phenol red, without calcium, without magnesium, liquid

SAFC

55021C

Hanks′ Balanced Salt solution

Hanks’ Balanced Salt solution Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

H9394

Hanks′ Balanced Salt solution

Sigma-Aldrich

Sigma-Aldrich

H8264

Hanks′ Balanced Salt solution

sterility

sterile-filtered

sterility

sterile

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: no

components

NaHCO3: 350 mg/L
phenol red: 10.62 mg/L
Hanks’ salts (2% CO2): yes

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L

components

phenol red: no
glucose: 1.0 g/L (Dextro)
NaHCO3: 0.35 g/L

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

General description

The essential function of Hanks′ Balanced Salt solution is to maintain pH as well as osmotic balance. It also provides water and essential inorganic ions to cells.
Formulation: click here.

Application

Hanks′ Balanced Salt solution has been used:

  • to study the degradation and cytotoxic effects of the composite material and the ion and/or magnetite release from the scaffolds.[1]
  • to measure the stability of the magnetic scaffold material in bone tissue engineering.[2]
  • in Fluorometric Measurements of intracellular calcium levels [Ca2+][3]
  • for isolating polymorphonuclear leukocytes (PMN) from umbilical cord blood[4]
  • For assessing airway inflammation in mice[5]

Other Notes

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when growing cells at low densities.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
RNBL8541
RNBL7700
RNBL6570
RNBL6573
RNBL6572

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A Tampieri et al.
Nanotechnology, 22(1), 015104-015104 (2010-12-08)
Magnetic bio-hybrid porous scaffolds have been synthesized, nucleating nano-apatite in situ on self-assembling collagen, in the presence of magnetite nano-particles. The magnetic phase acted as a sort of cross-linking agent for the collagen, inducing a chemico-physical-mechanical stabilization of the material
N Bock et al.
Acta biomaterialia, 6(3), 786-796 (2009-10-01)
In recent years, interest in tissue engineering and its solutions has increased considerably. In particular, scaffolds have become fundamental tools in bone graft substitution and are used in combination with a variety of bio-agents. However, a long-standing problem in the
Janhavi Sharma et al.
Journal of immunological methods, 351(1-2), 30-35 (2009-10-06)
One of the initial steps in the inflammatory process involves the adherence and transmigration of circulating polymorphonuclear leukocytes (PMN) across the endothelial cell monolayer. One of the main constituents of the neutrophil phagosome that contributes to bacterial killing is myeloperoxidase
Iliana Moreschi et al.
The Journal of biological chemistry, 281(42), 31419-31429 (2006-08-24)
Micromolar concentrations of extracellular beta-NAD+ (NAD(e)+) activate human granulocytes (superoxide and NO generation and chemotaxis) by triggering: (i) overproduction of cAMP, (ii) activation of protein kinase A, (iii) stimulation of ADP-ribosyl cyclase and overproduction of cyclic ADP-ribose (cADPR), a universal
Percíllia Victória Santos de Oliveira et al.
Redox biology, 22, 101142-101142 (2019-03-15)
Redox-related plasma proteins are candidate reporters of protein signatures associated with endothelial structure/function. Thiol-proteins from protein disulfide isomerase (PDI) family are unexplored in this context. Here, we investigate the occurrence and physiological significance of a circulating pool of PDI in
View All Related Papers

Protocols

Rat Dermal Fibroblasts (RDF) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Bovine Aortic Endothelial Cells (BAOEC) Culture Protocol

Technical information for working with bovine aortic endothelial cells including thawing, subculturing and cryopreservation.

Human Aortic Endothelial Cells (HAOEC) Culture Protocol

Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation.

Human Astrocytes (HA) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

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