All Photos(1)

H6875

Sigma-Aldrich

Hippuryl-L-phenylalanine

Synonym(s):
N-Benzoyl-Gly-Phe, Hippuryl-Phe
Empirical Formula (Hill Notation):
C18H18N2O4
CAS Number:
Molecular Weight:
326.35
Beilstein:
2167818
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

assay

≥98% (TLC)

Quality Level

form

powder

solubility

acetic acid: 50 mg/mL, clear, colorless

storage temp.

−20°C

SMILES string

OC(=O)[C@H](Cc1ccccc1)NC(=O)CNC(=O)c2ccccc2

InChI

1S/C18H18N2O4/c21-16(12-19-17(22)14-9-5-2-6-10-14)20-15(18(23)24)11-13-7-3-1-4-8-13/h1-10,15H,11-12H2,(H,19,22)(H,20,21)(H,23,24)/t15-/m0/s1

InChI key

CCLJGZGVIQBNDH-HNNXBMFYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

Hippuryl-L-phenylalanine has been used as a substrate for screening carboxypeptidase activity in Trogoderma granarium, Bactrocera oleae Gmelin and Apodiphus amygdali.

Biochem/physiol Actions

Hippuryl-L-phenylalanine is a substrate for carboxypeptidase A enzyme.

Substrates

Substrate for carboxypeptidase A

Storage Class Code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Product stabilization of the two forms of carboxypeptidase A from buffalo pancreas
Sud M and Dua RD
Journal of Biosciences, 9(1-2), 83-90 (1985)
Determination of kininase I and kininase II activities in human urine by high-performance liquid chromatography.
G Porcelli et al.
Journal of chromatography, 414(2), 423-428 (1987-03-06)
E Normant et al.
Neuropeptides, 30(1), 13-17 (1996-02-01)
We have designed two radioactive substrates, hippuryl-L-[3H]phenylalanine and 3-(p-hydroxy, m-[125I]phenyl)propionic acid ([125I]Bolton reagent) derivative of L-arginyl-L-phenylalanine, i.e. [125I]BRF, for a highly sensitive assay of carboxypeptidase A (CPA) activity. After cleavage of the C-terminal phenylalanine residue by CPA, the radioactive product...
Digestive proteolytic activity in larvae and adults of Bactrocera oleae Gmelin (Diptera: Tephritidae)
Delkash-Roudsari S, et al.
Journal of Asia-Pacific Entomology, 17(3), 483-491 (2014)
Mast cell proteases.
R G Woodbury et al.
Methods in enzymology, 80 Pt C, 588-609 (1981-01-01)

Protocols

Enzymatic Assay of Carboxypeptidase

Objective: To standardize a procedure for the assay of Carboxypeptidase A

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service