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H7016

Sigma-Aldrich

Hirudin from leeches

lyophilized powder, ≥1,500 ATU/mg protein (ATU = antithrombin units)

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CAS Number:
EC Number:
MDL number:

biological source

animal (Hirudo medicinalis)

Quality Level

100
200

form

lyophilized powder

specific activity

≥1,500 ATU/mg protein (ATU = antithrombin units)

mol wt

7 kDa

solubility

pyridine: soluble
water: soluble

storage temp.

2-8°C

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This Item
H0393M2272C7661
Sigma-Aldrich

Sigma-Aldrich

H7016

Hirudin from leeches

Sigma-Aldrich

Sigma-Aldrich

H0393

Hirudin

Collagen from rat tail Bornstein and Traub Type I, powder, BioReagent, suitable for cell culture

Sigma-Aldrich

C7661

Collagen from rat tail

form

lyophilized powder

form

powder

form

lyophilized powder

form

powder

mol wt

7 kDa

mol wt

~7 kDa

mol wt

~_2.8 kDa

mol wt

-

solubility

pyridine: soluble, water: soluble

solubility

pyridine: soluble, water: soluble

solubility

water: 5.00-5.20 mg/mL, clear, colorless to faintly yellow

solubility

soluble (Product is clear to hazy colorless solution with a few insolubles at 1 mg/ml in water with 2 μl acetic acid (or 0.1 N acetic acid). The insolubles can be removed by settling or centrifugation.)

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

100, 200

Quality Level

200

Quality Level

200

Quality Level

-

General description

Hirudin is a 7 kDa acidic protein containing 65 amino acid residues that has an isoelectric point of 3.5-4.0. It is not glycosylated and lacks tryptophan, arginine and methionine residues. It is chemically stable between pH 2-12 and at temperatures up to 80°C.

Biochem/physiol Actions

The anticoagulant, hirudin, is the most potent natural inhibitor of both soluble and clot-bound thrombin. It forms a high-affinity 1:1 complex with thrombin, occluding both the proteolytic site and exosite I (fibrinogen and PAR recognition site). Hirudin blocks thrombus growth and platelet activation, and has been suggested as the clinically preferred anticoagulant (over heparin and citrate) for its specific mode of action and absence of side effects. It is not metabolized in the bloodstream of humans and is eliminated unchanged via kidney filtration.

Unit Definition

One unit (ATU) will neutralize one NIH unit of thrombin at 37 °C, based on direct comparison to an NIH thrombin reference standard.

Analysis Note

Protein determined by Lowry.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Hanif Khan et al.
Chemical biology & drug design, 89(1), 67-73 (2016-08-03)
Hirudin is clinically an important drug used for the treatment of cardiac diseases, but has never been elucidated for antileishmanial potential. This study was designed to determine the therapeutic utility of hirudin against leishmaniasis. Binding affinities of 28 potent proteinase
Sarah E Sartain et al.
Journal of immunology (Baltimore, Md. : 1950), 196(2), 832-845 (2015-12-18)
Atypical hemolytic uremic syndrome (aHUS) is a thrombotic microangiopathy with severe renal injury secondary to an overactive alternative complement pathway (AP). aHUS episodes are often initiated or recur during inflammation. We investigated gene expression of the surface complement regulatory proteins
Mengzhou Xue et al.
Brain : a journal of neurology, 132(Pt 1), 26-36 (2008-09-06)
Proteases such as matrix metalloproteinases (MMPs) and thrombin are implicated in intracerebral haemorrhage (ICH) but their interactions amongst one another and interdependency remain to be defined. The latter is important since proteases acting through different mechanisms to inflict neurotoxicity would
Alexandra Vasilyeva et al.
Biomolecules, 10(6) (2020-06-21)
The blood coagulation factor XIII (FXIII) plays a critical role in supporting coagulation and fibrinolysis due to both the covalent crosslinking of fibrin polymers, rendering them resistant to plasmin lysis, and the crosslinking of fibrin to proteins of the fibrinolytic
Yaoming Wang et al.
Stroke, 40(5), 1864-1869 (2008-12-06)
Activated protein C (APC), a protease with anticoagulant and cytoprotective activities, protects neurons and endothelium from ischemic injury. Drotrecogin-alfa activated, a hyperanticoagulant form of human recombinant APC, is currently being studied in patients with ischemic stroke. How changes in APC

Protocols

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

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