H7041

Sigma-Aldrich

Interleukin-2 human

IL-2, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin tested

Synonym(s):
T-cell growth factor, Interleukin-2
MDL number:
NACRES:
NA.77

Quality Level

biological source

human

recombinant

expressed in HEK 293 cells

form

lyophilized powder

potency

≤5.0 ng/mL ED50

quality

endotoxin tested

mol wt

dimer 15 kDa (glycosylated)

packaging

pkg of 5X10 μg
pkg of 10 μg
pkg of 100 μg

application(s)

cell culture | mammalian: suitable

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... IL2(3558)

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General description

Interleukin-2 (IL-2) is an α-helical cytokine belonging to T-helper cell type 1. It has a molecular weight of 15kDa. The IL2 gene is mapped to human chromosome 4q27. IL-2 is originally called as T cell growth factor.

Application

Interleukin-2 human has been used to culture peripheral blood mononuclear cells (PBMC). It has been used to activate effector cell (PBMC) and to study antibody-dependent cell-mediated cytotoxicity.

Packaging

10 μg in poly bottle

Biochem/physiol Actions

Interleukin-2 (IL-2) is essential for proliferation of natural killer (NK) cells, lymphocytes and macrophages. It plays a key role in the immune system functions such as NK cell-stimulated immune responses. IL-2 also mediates the formation and essential functions of regulatory T cells. It is implicated in autoimmune disease. IL-2 is associated with apoptosis and thus, possesses antitumor response. IL-2 mediates CD41+ T-cells differentiation into T-helper cell 1 and 2 effector subsets, preventing T-helper 17 differentiation.
Interleukin-2 (IL-2) is an immunomodulatory factor produced by certain subsets of T lymphocytes. This lympho­kine promotes long term growth of acti­vated T cells and related cell types. IL-2 plays a role in the activation and proliferation of NK cells, induces γ-inter­feron and B cell growth factor secretion, and modulates the expression of the IL-2 receptor.

Preparation Note

Human Interleukin-2 (IL-2) expressed in human HEK 293 cells is a glycosylated monomer with a molecular mass of 15 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 cells (mouse cytotoxic T cell line).

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Loss of immune tolerance to IL-2 in type 1 diabetes
Perol L, et al.
Nature Communications, 7, 13027-13027 (2016)
N-glycan engineering of a plant-produced anti-CD20-hIL-2 immunocytokine significantly enhances its effector functions
Marusic C, et al,
Biotechnology and Bioengineering, 115(3), 565-576 (2018)
Interleukin 2, 37-37 (2012)
Functional incompatibility between the generic NF-? motif and a subtype-specific Sp1III element drives the formation of HIV-1 subtype C viral promoter
Verma A, et al.
Journal of Virology, JVI-00308 (2016)
Association of- 330 interleukin-2 gene polymorphism with oral cancer
Singh PK, et al.
The Indian Journal of Medical Research, 146(6), 730-730 (2017)

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