H7291

Interleukin-4 human

potency:, IL-4, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture

• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00151705
• Biological source: human
• Recombinant: expressed in HEK 293 cells
• Assay: >95% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: dimer 14-19 kDa (glycosylated)
• Impurities: <1 EU/μg

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• assay: >95% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤0.6 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: dimer 14-19 kDa (glycosylated)
• packaging: pkg of 1 mg, pkg of 10 μg, pkg of 100 μg
• manufacturer/tradename: HumanZyme
• technique(s): cell culture | mammalian: suitable
• impurities: <1 EU/μg
• UniProt accession no.: P05112
• storage temp.: −20°C
• Quality Level: 100
• Gene Information: human ... IL4(3565)

Description

General description

Interleukin-4 (IL-4) is a pleiotropic cytokine[1] and a complex glycoprotein[2] mapped to human chromosome 5q31.1.[1] It is produced by mast cells, basophils and a subset of activated T cells.[2]

Biochem/physiol Actions

Interleukin-4 (IL-4) interacts with interleukin 13 and is an interleukin-4 receptor ligand.[1] It regulates IgE and IgG1 production by B cells.[3] IL-4 favors glial and neuronal differentiation[4] and regulates differentiation of naive cluster of differentiation CD4⁺ T cells to the T helper type 2 (TH2) cells.[5] Polymorphism in the IL-4 gene is implicated in immune and inflammatory disorders.[1] In cerebral infarction, IL-4 levels are elevated.[1]

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Preparation Note

HumanKine Interleukin-4 (IL-4), expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 14-19 kDa due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The activity is determined by the dose-dependent simulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable