H7411

Anti-HA−FITC antibody, Mouse monoclonal

clone HA-7, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin
• MDL number: MFCD00803873
• NACRES: NA.56

Properties

• Quality Level: 200
• biological source: mouse
• conjugate: FITC conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: HA-7, monoclonal
• form: buffered aqueous solution
• storage condition: protect from light
• concentration: 1 mg/mL
• technique(s): immunocytochemistry: 1-5 μg/mL using HA tagged fusion protein transfected into mammalian cells fixed with paraformaldehyde.
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Anti HA-FITC is a mouse monoclonal antibody derived from the HA-7 hybridoma. It is isolated from ascites fluid and conjugated to fluorescein isothiocyanate, isomer I (FITC). The antibody recognizes the HA tag expressed on the N- or C-terminus on HA tagged H7411proteins. The antibody reacts specifically with HA tagged proteins by immunocytochemistry.

Hemagglutinin (HA) is the major envelope glycoprotein of the influenza virus, along with neuraminidase.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Application

Antibody suitable for Immunofluorescence. Recommended concentration 1-5 mg/ml. For immunocytochemistry, use HA tagged fusion protein transfected into mammalian cells, then fixed with paraformaldehyde. Anti-HA-FITC antibody, Mouse monoclonal has been used:

• in the labeling of HA-tagged transient receptor potential melastatin 7 (TRPM7) channel protein for immunocytochemistry studies in lymphoblast cell DT40 and human embryonic kidney cells (HEK-293) cells
• in western blotting detection of spindle-associated proteins in Trypanosoma brucei
• in immunostaining of vascular smooth muscle cells (SMCs) for immunocytofluorescence studies

Biochem/physiol Actions

Hemagglutinin (HA) induces immune response post viral infection. Fluorescein isothiocyanate (FITC) conjugated with the influenza virus HA is majorly used in intracellular tracking of the virus and also in flow cytometric studies.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable