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H7416

Sigma-Aldrich

Interleukin-6 human

IL-6, recombinant, expressed in HEK 293 cells, suitable for cell culture

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Synonym(s):
IL-6
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in HEK 293 cells

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

≤2.0 ng/mL ED50

quality

endotoxin tested

mol wt

dimer 26-30 kDa (glycosylated)

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1.00 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... IL6(3569)

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This Item
SCU0001I139511138600001
Interleukin-6 human IL-6, recombinant, expressed in HEK 293 cells, suitable for cell culture

Sigma-Aldrich

H7416

Interleukin-6 human

Interleukin-6 human recombinant, expressed in HEK 293 cells, suitable for cell culture

Sigma-Aldrich

SCU0001

Interleukin-6 human

Interleukin-6 human IL-6, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sigma-Aldrich

I1395

Interleukin-6 human

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

>98% (SDS-PAGE)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

solution

potency

≤2.0 ng/mL ED50

potency

0.2-2 ng/mL EC50

potency

0.2-2.0 ng/mL ED50/EC50

potency

<0.01 ng/mL EC50

mol wt

dimer 26-30 kDa (glycosylated)

mol wt

calculated mol wt 20.8 kDa (183 amino acids glycosylated protein)

mol wt

26 kDa

mol wt

20,600 Da

General description

The IL6 (interleukin-6) gene is mapped to human chromosome 7p15.3. All the members of IL6 family have membrane glycoprotein gp130 as a common receptor. IL6 (interleukin-6) induces B-cell differentiation and is a primary regulator of inflammation.

Application

Interleukin-6 human has been used to check its role in breast cancer metastasis. It has also been used for evaluating its role in D5HS tumor sphere formation.

Biochem/physiol Actions

IL6 regulates tumor proliferation and inhibits apoptosis. It contributes to the disease progression of coronary artery disease.
Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Preparation Note

Human IL-6, expressed in human 293 cells, is a glycosylated monomer with an apparent molecular mass of 26-30 kDa due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Generation of Prostate Tumor-initiating Cells is Associated with Elevation of Reactive Oxygen Species and IL6/STAT3 Signaling
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Castellana B, et al.
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Scheller J, et al.
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The-174 G> C interleukin-6 gene polymorphism is associated with angiographic progression of coronary artery disease over a 4-year period.
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