BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
Beilstein/REAXYS Number:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

product line



≥99.5% (titration)




Insoluble matter, passes filter test

ign. residue (900 °C)

≤0.1% (as SO4)


≤1.0% loss on drying, 110°C


5.0-6.5 (1 M in H2O)

useful pH range

6.8 - 8.2

pKa (25 °C)



H2O: 1 M, clear, colorless

anion traces

chloride (Cl-): ≤0.05%

cation traces

Al: ≤0.0005%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.001%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.005%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%


≤0.035 at 280 in H2O at 1 M
≤0.045 at 260 in H2O at 1 M

Featured Industry

Diagnostic Assay Manufacturing

SMILES string




InChI key


Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.


HEPES has been used:
  • As a component of homogenisation buffer used in protein sample preparation
  • To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation
  • As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation
  • As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.


50, 250 g in poly bottle

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

The inhibitory effect of intracerebroventricularly injected interleukin 1? on testosterone secretion in the rat: role of steroidogenic acute regulatory protein.
Ogilvie KM, et al.
Biology of Reproduction, 60(2), 527-533 (1999)
In vitro tubulogenesis of endothelial cells by relaxation of the coupling extracellular matrix-cytoskeleton.
Deroanne CF, et al.
Cardiovascular Research, 49(3), 647-658 (2001)
Intervertebral disc regeneration in an ex vivo culture system using mesenchymal stem cells and platelet-rich plasma.
Chen WH, et al.
Biomaterials, 30(29), 5523-5533 (2009)
Hsp60-induced tolerance in the rotifer Brachionus plicatilis exposed to multiple environmental contaminants.
Wheelock CE, et al.
Archives of Environmental Contamination and Toxicology, 36(3), 281-287 (1999)
Increased expression of reticulon 3 in neurons leads to reduced axonal transport of $\beta$ site amyloid precursor protein-cleaving enzyme 1.
Deng M, et al.
The Journal of Biological Chemistry, 288(42), 30236-30245 (2013)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.