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MilliporeSigma

H7782

Hybridization solution

Synonym(s):

Hyb solution, Hybridization solution for in situ hybridization, Ready-to-use hybridization solution

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6 ML

$116.00

$116.00


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About This Item

UNSPSC Code:
12161503
EC Number:
200-842-0
NACRES:
NA.32

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This Item
H7140S4040H7033
technique(s)

FISH: suitable

technique(s)

-

technique(s)

Northern blotting: suitable, Southern blotting: suitable

technique(s)

-

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

form

liquid

form

liquid (2x concentrate)

form

-

form

solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

room temp

Application

Hybridization solution has been used in:
  • fluorescent in situ hybridization (FISH) to perform probe annealing[1]
  • in situ hybridization (ISH) to dilute probe for the detection of amastigote forms of Leishmania infantum[2]
  • FISH analysis to hybridize probes[3]

General description

In situ hybridization assays detect a specific nucleic acid sequence of a gene at the cellular level. Hybridization solution is used to enhance hybrid formation between the DNA probe and the target mRNA on tissue sections. It may be used for reconstituting and/or diluting the probe for in situ hybridization experiments, or as a negative control (without probe).
Ready-to-use hybridization solution optimized for in situ hybridization (ISH) procedures.

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

target_organs

Blood

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Detection of characteristic heterochromatin distribution, highly amplified rRNA genes and presence of the human satellite III DNA motif in the scleractinian coral Echinophyllia aspera Ellis and Solander 1788
Taguchi T, et al.
Chromosome Analysis Protocols, 16, 33-38 (2013)
Ying Lv et al.
Journal of the American Heart Association, 8(2), e010456-e010456 (2019-01-15)
Background Intrauterine growth retardation ( IUGR ) is related to pulmonary artery hypertension in adults, and mi croRNA -206 (miR-206) is proposed to affect the proliferation and apoptosis of pulmonary artery smooth muscle cells ( PASMC s) via post-transcriptional regulation.
Aurea V A da Silva et al.
Immunity, inflammation and disease, 8(2), 127-139 (2020-03-25)
In canine visceral leishmaniasis (CVL), lymphopenia, and the disorganization of lymphoid organs such as spleen and lymph nodes have been demonstrated. However, the involvement of thymus in CVL has not been evaluated so far. Herein, we investigated whether the thymus
Melanie J Harriff et al.
Journal of bacteriology, 191(4), 1132-1142 (2008-12-09)
The Mycobacterium avium complex (MAC) is an important group of opportunistic pathogens for birds, cattle, swine, and immunosuppressed humans. Although invasion of epithelial cells lining the intestine is the chief point of entry for these organisms, little is known about

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Questions

1–3 of 3 Questions  
  1. Is this buffer appropriate for Fluorescent in situ hybridization using probes labeled with ATTO by nick translation?

    1 answer
    1. This product has not been tested for the application. So, there is no reference data available.

      Helpful?

  2. Is this buffer appropriate to use with all probe types? Will it work with labeled (DIG, DNP, Biotin, etc.) polynucleotide probes?

    1 answer
    1. The buffer is optimized for both DNA and RNA probes for in situ hybridization. It has been used with FITC and biotinylated probes.

      Helpful?

  3. What is the composition of the Hybridization solution ( H7782)?

    1 answer
    1. The specific formulation of this product is considered proprietary but includes dextran sulfate and formamide in a sodium citrate solution.

      Helpful?

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