Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

H8032

Hanahan′s Broth (SOB Medium)

powder microbial growth medium

Synonym(s):

Super Optimal Broth

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice
500 g

Available to ship TODAYfromMILWAUKEE

$200.00

About This Item

NACRES:
NA.85
UNSPSC Code:
41106200

$200.00

Available to ship TODAYDetails

​
​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

grade

Molecular Biology

Quality Level

100

description

recommended for cultivation of recombinant E. coli.

sterility

non-sterile

form

powder

composition

KCl, 0.186 g/L , MgSO4, 2.4 g/L , NaCl, 0.5 g/L , Tryptone, 20 g/L , Yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

pH

6.8-7.2(2.8% solution)

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

General description

Hannahan’s Broth (SOB) is a microbial growth medium used for the preparation and transformation of competent cells (E. coli). Casein enzymic hydrolysate and yeast extract supply nitrogenous compounds and growth factors for recombinant Escherichia coli. Potassium chloride and sodium chloride maintain the isotonic condition. Magnesium sulfate is added to the medium as a necessary component for DNA replication.

Application

Hanahan′s Broth is recommended for use in cultivation of recombinant strains of Escherichia coli. It is suitable for the preparation of competent E. coli., often with the addition of 0.4% dextrose. It has also been used for the preparation of C. necator H16 competent cells. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.

Features and Benefits

Hannahan′s Broth (SOB) powder provides:
  • Standard formulation
  • An inexpensive alternative to liquid

Preparation Note

Stir to suspend 28 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Other Notes

Casein enzymic hydrolysate and yeast extract supply the necessary nitrogenous compounds and growth factors. Potassium chloride and sodium chloride provide an isotonic environment for the cells. Magnesium sulfate aids DNA replication.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
L7533L3522L3022
Hanahan’s Broth (SOB Medium) powder microbial growth medium

Sigma-Aldrich

H8032

Hanahan′s Broth (SOB Medium)

LB Broth with agar (Lennox) EZMix powder microbial growth medium

Sigma-Aldrich

L7533

LB Broth with agar (Lennox)

LB Broth (Miller) Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture

Sigma-Aldrich

L3522

LB Broth (Miller)

LB Broth (Lennox) Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

Sigma-Aldrich

L3022

LB Broth (Lennox)

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

-

technique(s)

microbiological culture: suitable

form

powder

form

powder

form

powder

form

powder

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

composition

KCl, 0.186 g/L , NaCl, 0.5 g/L , Yeast extract, 5 g/L , MgSO4, 2.4 g/L , Tryptone, 20 g/L

composition

Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

composition

NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

composition

NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

application(s)

food and beverages
microbiology

application(s)

agriculture
food and beverages
microbiology

application(s)

food and beverages

application(s)

food and beverages
microbiology

Still not finding the right product?

Explore all of our products under Hanahan′s Broth (SOB Medium)

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
MKCZ3514
MKCV5945
MKCW9976
MKCS6546
MKCQ5868

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Related Content

Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16
Arenas-LC, et al.
Biotechnology for Biofuels, 12, 150-150 (2019)
Nicole Pearcy et al.
PLoS computational biology, 18(5), e1010106-e1010106 (2022-05-24)
Exploiting biological processes to recycle renewable carbon into high value platform chemicals provides a sustainable and greener alternative to current reliance on petrochemicals. In this regard Cupriavidus necator H16 represents a particularly promising microbial chassis due to its ability to
View All Related Papers

Global Trade Item Number

SKUGTIN
H8032-500G04061833805008

Questions

1–2 of 2 Questions  

  1. Which bacterial culture medium is the best choice for my application?

    1 answer

    1. Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

Reviews

No rating value

Active Filters