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MilliporeSigma

H8280

Sigma-Aldrich

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow

matrix fast-flow highly cross-linked 4% beaded agarose

Synonym(s):

N-Hydroxysuccinimidyl-Agarose

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About This Item

MDL number:
MFCD00164950
UNSPSC Code:
23151817
NACRES:
NA.56

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form

isopropanol suspension

Quality Level

200

extent of labeling

16-23 μmol per mL

technique(s)

protein array: suitable

matrix

fast-flow highly cross-linked 4% beaded agarose

matrix active group

N-hydroxysuccinimide active ester

matrix spacer

6 atoms (when ligands are coupled by displacement of NHS)

storage temp.

2-8°C

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This Item
C5338GE17-0906-01P7892
N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow matrix fast-flow highly cross-linked 4% beaded agarose

Sigma-Aldrich

H8280

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow

Cyanogen bromide-activated-Sepharose™ 4 Fast Flow lyophilized powder

Sigma-Aldrich

C5338

Cyanogen bromide-activated-Sepharose™ 4 Fast Flow

NHS Act Sepharose™ 4 Fast Flow Cytiva 17-0906-01, pack of 25 mL

GE17-0906-01

NHS Act Sepharose™ 4 Fast Flow

Phenyl-Sepharose CL-4B extent of labeling: ~40 μmol per mL

Sigma-Aldrich

P7892

Phenyl-Sepharose CL-4B

matrix active group

N-hydroxysuccinimide active ester

matrix active group

cyanate or related structures

matrix active group

-

matrix active group

-

matrix

fast-flow highly cross-linked 4% beaded agarose

matrix

Sepharose 4 Fast Flow

matrix

4% cross-linked agarose

matrix

agarose, 4% cross-linked

form

isopropanol suspension

form

lyophilized powder

form

-

form

suspension

technique(s)

protein array: suitable

technique(s)

affinity chromatography: suitable

technique(s)

-

technique(s)

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

200

General description

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow is a cross-linked and preactivated matrix obtained by mixing Sepharose 4 Fast Flow with 6-aminohexanoic acid through a spacer arm. Esterification with N-hydroxysuccinimide activates the terminal carboxyl group. Ligand-containing primary amino groups directly interact with this active ester to form a chemically very stable amide linkage.

Application

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow has been used as a part of a magnetic particles-based visual detection system to achieve separation upon binding of the amplified gene. It has also been used to immobilize protein during pull-down assay.

Features and Benefits

  • Specifically recommended for coupling of small amino-containing proteins and peptides in process-scale applications
  • High level of pre-activation, which gives a high degree of substitution of the selected ligand
  • NHS coupling method enables chemically stable ligand attachment

Legal Information

Sepharose is a trademark of Cytiva

pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

53.6 °F

flash_point_c

12 °C

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
0000389216
0000389216
0000335729
SLCM2411
SLCH4912

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Woo-Hyeon Byeon et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(2), 361-363 (2004-05-12)
Combinatorial phage display was used to discover peptides that selectively bind to the alpha-cobratoxin (neurotoxin) component of the multi-component venom of the Thai cobra, Naja kaouthia. Peptide sequences determined in this way were synthesized chemically and were covalently attached to
Yoo Min Park et al.
Talanta, 195, 97-102 (2019-01-11)
The current study focuses on developing a system for visually detecting an amplified bacterial (Escherichia coli O157:H7) gene using a heavy metal particle (MP) and functionalized porous sepharose gel. To functionalize DNA-specificity to the MP, an avidin-modified MP was employed
Seulggie Choi et al.
Molecular immunology, 48(15-16), 2207-2213 (2011-07-12)
Porphyromonas gingivalis causes periodontal diseases and its lipopolysaccharide (LPS) is considered as a major virulence factor responsible for pathogenesis. Since initial recognition of P. gingivalis LPS (Pg.LPS) in the oral cavity might be crucial for the host response, we identified
Yoo Min Park et al.
Biosensors & bioelectronics, 141, 111415-111415 (2019-06-16)
We focused on the development of a hand-held pathogen-detection device using smartphone-embedded electronic elements combined with functionalized magnetic particles (MPs) and sepharose. To perform affinity chromatography for evaluating DNA amplicons, avidin-conjugated MPs and succinimide-linked sepharose were used with biotin-primers. To
Julia V Hartig et al.
Nucleic acids research, 39(9), 3836-3851 (2011-01-20)
In Drosophila, siRNAs are classified as endo- or exo-siRNAs based on their origin. Both are processed from double-stranded RNA precursors by Dcr-2 and then loaded into the Argonaute protein Ago2. While exo-siRNAs serve to defend the cell against viruses, endo-siRNAs
View All Related Papers

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