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H8280

Sigma-Aldrich

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow

matrix fast-flow highly cross-linked 4% beaded agarose

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Synonym(s):
N-Hydroxysuccinimidyl-Agarose
MDL number:
MFCD00164950

form

isopropanol suspension

Quality Level

200

extent of labeling

16-23 μmol per mL

matrix

fast-flow highly cross-linked 4% beaded agarose

matrix active group

N-hydroxysuccinimide active ester

matrix spacer

6 atoms (when ligands are coupled by displacement of NHS)

storage temp.

2-8°C

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This Item
GE17-0906-01GE17-5132-03GE17-5132-01
N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow matrix fast-flow highly cross-linked 4% beaded agarose

Sigma-Aldrich

H8280

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow

NHS Act Sepharose™ 4 Fast Flow Cytiva 17-0906-01, pack of 25 mL

GE17-0906-01

NHS Act Sepharose™ 4 Fast Flow

Glutathione Sepharose™ 4 Fast Flow Cytiva 17-5132-03, pack of 500 mL

GE17-5132-03

Glutathione Sepharose™ 4 Fast Flow

Glutathione Sepharose™ 4 Fast Flow Cytiva 17-5132-01, pack of 25 mL

GE17-5132-01

Glutathione Sepharose™ 4 Fast Flow

matrix

fast-flow highly cross-linked 4% beaded agarose

matrix

4% cross-linked agarose

matrix

highly cross-linked 4% agarose

matrix

highly cross-linked 4% agarose

matrix active group

N-hydroxysuccinimide active ester

matrix active group

-

matrix active group

-

matrix active group

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

-

extent of labeling

16-23 μmol per mL

extent of labeling

-

extent of labeling

-

extent of labeling

-

matrix spacer

6 atoms (when ligands are coupled by displacement of NHS)

matrix spacer

-

matrix spacer

-

matrix spacer

-

General description

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow is a cross-linked and preactivated matrix obtained by mixing Sepharose 4 Fast Flow with 6-aminohexanoic acid through a spacer arm. Esterification with N-hydroxysuccinimide activates the terminal carboxyl group. Ligand-containing primary amino groups directly interact with this active ester to form a chemically very stable amide linkage.

Application

N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow has been used as a part of a magnetic particles-based visual detection system to achieve separation upon binding of the amplified gene. It has also been used to immobilize protein during pull-down assay.

Features and Benefits

  • Specifically recommended for coupling of small amino-containing proteins and peptides in process-scale applications
  • High level of pre-activation, which gives a high degree of substitution of the selected ligand
  • NHS coupling method enables chemically stable ligand attachment

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225 - H319 - H336

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

53.6 °F

Flash Point(C)

12 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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25G
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Cyanogen bromide-activated-Sepharose™ 4 Fast Flow lyophilized powder

Sigma-Aldrich

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Cyanogen bromide-activated-Sepharose™ 4 Fast Flow

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Cyanogen bromide-activated Agarose lyophilized powder

Sigma-Aldrich

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Woo-Hyeon Byeon et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(2), 361-363 (2004-05-12)
Combinatorial phage display was used to discover peptides that selectively bind to the alpha-cobratoxin (neurotoxin) component of the multi-component venom of the Thai cobra, Naja kaouthia. Peptide sequences determined in this way were synthesized chemically and were covalently attached to
Yoo Min Park et al.
Talanta, 195, 97-102 (2019-01-11)
The current study focuses on developing a system for visually detecting an amplified bacterial (Escherichia coli O157:H7) gene using a heavy metal particle (MP) and functionalized porous sepharose gel. To functionalize DNA-specificity to the MP, an avidin-modified MP was employed
Seulggie Choi et al.
Molecular immunology, 48(15-16), 2207-2213 (2011-07-12)
Porphyromonas gingivalis causes periodontal diseases and its lipopolysaccharide (LPS) is considered as a major virulence factor responsible for pathogenesis. Since initial recognition of P. gingivalis LPS (Pg.LPS) in the oral cavity might be crucial for the host response, we identified
Yoo Min Park et al.
Biosensors & bioelectronics, 141, 111415-111415 (2019-06-16)
We focused on the development of a hand-held pathogen-detection device using smartphone-embedded electronic elements combined with functionalized magnetic particles (MPs) and sepharose. To perform affinity chromatography for evaluating DNA amplicons, avidin-conjugated MPs and succinimide-linked sepharose were used with biotin-primers. To
Julia V Hartig et al.
Nucleic acids research, 39(9), 3836-3851 (2011-01-20)
In Drosophila, siRNAs are classified as endo- or exo-siRNAs based on their origin. Both are processed from double-stranded RNA precursors by Dcr-2 and then loaded into the Argonaute protein Ago2. While exo-siRNAs serve to defend the cell against viruses, endo-siRNAs
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