H8416

Sigma-Aldrich

Stem Cell Factor human

SCF, recombinant, expressed in HEK 293 cells, suitable for cell culture

Synonym(s):
SCF, c-Kit ligand
MDL number:
NACRES:
NA.77

Quality Level

biological source

human

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

≤200 ng/mL ED50

quality

endotoxin tested

mol wt

dimer 35-45 kDa (glycosylated)

packaging

pkg of 5x10 μg
pkg of 10 μg
pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

application(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... KITLG(4254)

Looking for similar products? Visit Product Comparison Guide

General description

The KITLG (KIT ligand) gene is mapped to human chromosome 12q21.32.

Packaging

10 μg in poly bottle

Biochem/physiol Actions

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.
Stem cell factor (SCF) of granulosa cell origin, efficiently contributes to the follicular development and oocyte maturation. SCF controls tumor development and progression. Elevated SCF gene expression is observed in hepatocellular carcinoma.

Preparation Note

SCF is expressed in human HEK 293 cells as a glycosylated monomer with an apparent molecular mass of 35-45 kDa due to glycosylation. Production in human 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Gene mapping study for constitutive skin color in an isolated Mongolian population
Paik SH, et al.
Experimental & Molecular Medicine, 44, 241-249 (2012)
Increased SCF in Follicular Fluid and Granulosa Cells Positively Correlates With Oocyte Maturation, Fertilization, and Embryo Quality in Humans.
Tan J, et al.
Reproductive Sciences, 24(11), 1544-1550 (2017)
HIF-2-dependent expression of stem cell factor promotes metastasis in hepatocellular carcinoma
Wang X, et al.
Cancer Letters, 393, 113-124 (2017)
Mireille-Maria Suttle et al.
Acta dermato-venereologica, 95(5), 536-541 (2014-11-27)
A slight epidermal damage can induce the Köbner reaction in psoriasis, and the “alarmin”, interleukin-33 (IL-33), may be involved in this process. Therefore, the uninvolved psoriatic skin was tape-stripped, and skin biopsies were collected at 0 day, 2 h and...
Yoshimichi Okayama et al.
Immunologic research, 34(2), 97-115 (2006-06-09)
Mast cells play a pivotal role in immediate hypersensitivity and chronic allergic reactions that can contribute to asthma, atopic dermatitis, and other allergic diseases. Because mast cell numbers are increased at sites of inflammation in allergic diseases, pharmacologic intervention into...
Articles
Read article on hematopoietic cytokines and hematopoiesis
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.