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H8416

Sigma-Aldrich

Stem Cell Factor human

SCF, recombinant, expressed in HEK 293 cells, suitable for cell culture

Synonym(s):

SCF, c-Kit ligand

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

≤200 ng/mL ED50

quality

endotoxin tested

mol wt

dimer 35-45 kDa (glycosylated)

packaging

pkg of 5x10 μg
pkg of 10 μg
pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... KITLG(4254)

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General description

The KITLG (KIT ligand) gene is mapped to human chromosome 12q21.32.

Biochem/physiol Actions

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.
Stem cell factor (SCF) of granulosa cell origin, efficiently contributes to the follicular development and oocyte maturation. SCF controls tumor development and progression. Elevated SCF gene expression is observed in hepatocellular carcinoma.

Preparation Note

SCF is expressed in human HEK 293 cells as a glycosylated monomer with an apparent molecular mass of 35-45 kDa due to glycosylation. Production in human 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Gene mapping study for constitutive skin color in an isolated Mongolian population
Paik SH, et al.
Experimental & Molecular Medicine, 44, 241-249 (2012)
Increased SCF in Follicular Fluid and Granulosa Cells Positively Correlates With Oocyte Maturation, Fertilization, and Embryo Quality in Humans.
Tan J, et al.
Reproductive Sciences, 24(11), 1544-1550 (2017)
HIF-2-dependent expression of stem cell factor promotes metastasis in hepatocellular carcinoma
Wang X, et al.
Cancer Letters, 393, 113-124 (2017)
Mireille-Maria Suttle et al.
Acta dermato-venereologica, 95(5), 536-541 (2014-11-27)
A slight epidermal damage can induce the Köbner reaction in psoriasis, and the “alarmin”, interleukin-33 (IL-33), may be involved in this process. Therefore, the uninvolved psoriatic skin was tape-stripped, and skin biopsies were collected at 0 day, 2 h and
Sibylle A Brenner et al.
Immunology, 143(2), 174-183 (2014-04-05)
Mast cells are now considered sentinels in immunity. Given their location underneath the gastrointestinal barrier, mast cells are entrusted with the task of tolerating commensal microorganisms and eliminating potential pathogens in the gut microbiota. The aim of our study was

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