H8509

β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides

Name: β-Hydroxybutyrate Dehydrogenase from <I>Rhodopseudomonas sphaeroides</I>
Brand: SIGMA
Price: 1560 USD

Type V, lyophilized powder, 250-750 units/mg protein

Synonym(s):

(R)-3-Hydroxybutanoate:NAD⁺ oxidoreductase, 3-HBDH

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25 UNITS

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About This Item

CAS Number:

9028-38-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-830-6

MDL number:

MFCD00130633

EC Number:

1.1.1.30 (BRENDA, IUBMB)

Key Documents

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Product Name

β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides, Type V, lyophilized powder, 250-750 units/mg protein

type

Type V

form

lyophilized powder

specific activity

250-750 units/mg protein

purified by

chromatography

foreign activity

lactate dehydrogenase ≤0.05%
malate dehydrogenase ≤0.1%

storage temp.

−20°C

Quality Level

200

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This Item	H9408	L3888	G6880
Sigma-Aldrich H8509 β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides	Sigma-Aldrich H9408 β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei	Sigma-Aldrich L3888 D-Lactic Dehydrogenase from Lactobacillus leichmannii	Sigma-Aldrich G6880 α-Glycerophosphate Dehydrogenase from rabbit muscle
specific activity 250-750 units/mg protein	specific activity ≥200 units/mg protein	specific activity 150-500 units/mg protein	specific activity ≥100 units/mg protein
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
foreign activity lactate dehydrogenase ≤0.05%, malate dehydrogenase ≤0.1%	foreign activity lactic dehydrogenase ≤0.05%, malic dehydrogenase ≤0.1%	foreign activity Malic dehydrogenase <0.5% of base activity	foreign activity Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%, Triosephosphate isomerase ≤0.02%
purified by chromatography	purified by chromatography	purified by -	purified by -

Application

Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).

Biochem/physiol Actions

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Rhodopseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).

Other Notes

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.

Physical form

Lyophilized powder containing Tris buffer salts

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Resting and post-exercise serum biomarkers of cardiac and skeletal muscle damage in adolescent runners.

J Nie et al.

Scandinavian journal of medicine & science in sports, 21(5), 625-629 (2010-05-13)

This study examined the response of serum biomarkers of cardiac and skeletal muscle damage at rest and after a routine workout of 21 km run in 12 male adolescent (16.2±0.6 years) long-distance runners. Biomarkers of cardiac [troponins (cTnT, cTnI), creatine

An enzymatic method to determine γ-hydroxybutyric acid in serum and urine.

Lara Hasan et al.

Therapeutic drug monitoring, 33(6), 757-765 (2011-11-23)

Gamma-hydroxybutyric acid (GHB) has become one of the most dangerous illicit drugs of abuse today. It is used as a recreational and date rape drug because of its depressant effect on the central nervous system, which may cause euphoria, amnesia

Development and characterization of an enzymatic method for the rapid determination of gamma hydroxybutyric acid.

Michel A Sciotti et al.

Chimia, 64(11), 793-798 (2011-01-05)

Gamma hydroxybutyric acid (GHB) is a regulated therapeutic drug, which naturally occurs in mammalian brain tissues as an intermediate of the GABA (gamma aminobutyric acid) neurotransmitter metabolism. The increasing misuse of GHB as a narcotic or abusing drug in recent

Identification of succinic semialdehyde reductases from Geobacter: expression, purification, crystallization, preliminary functional, and crystallographic analysis.

Yanfeng Zhang et al.

Acta biochimica et biophysica Sinica, 43(12), 996-1002 (2011-11-01)

Succinic semialdehyde reductase (SSAR) is an important enzyme involved in γ-aminobutyrate (GABA) metabolism. By converting succinic semialdehyde (SSA) to γ-hydroxybutyrate (GHB), the SSAR facilitates an alternative pathway for GABA degradation. In this study, we identified SSARs from Geobacter sulfurreducens and

Inborn errors of ketogenesis and ketone body utilization.

Jörn Oliver Sass

Journal of inherited metabolic disease, 35(1), 23-28 (2011-04-12)

Ketone bodies acetoacetate and 3-hydroxy-n-butyric acid are metabolites derived from fatty acids and ketogenic amino acids such as leucine. They are mainly produced in the liver via reactions catalyzed by the ketogenic enzymes mitochondrial 3-hydroxy-3-methylglutary-coenzyme A synthase and 3-hydroxy-3-methylglutary-coenzyme A

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β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides