This product should be reconstituted in sterile 4 mM HCl at 100ug/mL or greater. For stock concentrations of less than 100 ug/mL, the addition of 0.1% low endotoxin albumin is recommended. Prepared solutions may be stored at 2–8 °C for several days or in working aliquots at –20 °C to –80 °C for long term. Avoid repeated freeze-thaw cycles. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/410/926/h8541-pis-ms.pdf
H8541
Transforming Growth Factor-β1 human
≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Synonym(s):
TGF-β1
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Product Name
Transforming Growth Factor-β1 human, TGF-β1, Xeno-free, recombinant, expressed in HEK 293 cells, suitable for cell culture
biological source
human
Quality Level
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤0.5 ng/mL EC50
quality
endotoxin tested
mol wt
dimer 25 kDa (non-glycosylated)
packaging
pkg of 5 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg
UniProt accession no.
storage temp.
-10 to -25°C
Gene Information
human ... TGFB1(7040)
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1 of 4
This Item | T2815 | T5300 | L3408 |
|---|---|---|---|
| assay ≥95% (SDS-PAGE) | assay ≥97% (electrophoresis) | assay ≥97% (SDS-PAGE and N-terminal analysis) | assay ≥97% (SDS-PAGE) |
| biological source human | biological source human | biological source Porcine (Platelets) | biological source human |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| recombinant expressed in HEK 293 cells | recombinant expressed in NSO cells | recombinant - | recombinant expressed in Sf21 cells |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form lyophilized powder | form powder | form powder | form lyophilized powder |
General description
The TGFB1 gene is mapped to human chromosome 19q13.2 and codes for a 25 kDa peptide.[1][2] TGF-ß1 polypeptide is produced as a precursor with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The biologically active form of Tgfb1 exists as a disulphide-linked homodimer.[3]
Application
Transforming Growth Factor-β1 (TGF-β) human has been used :
- to study the expression of PDGFRB (Platelet derived growth factor receptor β) in association with transforming growth factor-β signaling.[4]
- to stimulate lung fibrosis in human fetal lung fibroblast cell line in order to further measure the in-plane elasticity of live cell layers using a pressure sensor embedded microfluidic device.[5]
- to study the effect of TGF-β on HTRA1 (high-temperature requirement A1)-affected Wnt target genes.[6]
Biochem/physiol Actions
TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types.[2] Transformed as well as non-neoplastic tissues release transforming growth factors and essentially all cells possess a specific TGF-β1 receptor. In general, cells of mesenchymal origin appear to be stimulated by TGF-β1; whereas, hepatocytes, T and B lymphocytes, keratinocytes, and many epithelial cells are inhibited by the peptide. TGF-β1 interacts with Epidermal Growth Factor (EGF), Platelet Derived Growth Factor (PDGF), Fibroblast Growth Factor (FGF), and T Cell Growth Factor (T-CGF) either by enhancing or antagonizing their characteristic actions. TGF-β1 plays a fundamental role in tissue growth and differentiation by involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation, and immune cell function.[7] TGF-β1 is associated with the developmental and metastasis of colorectal cancer.[8]
Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.
Physical form
Lyophilized from a 0.2 μm filtered solution of 50 mM NaOAc pH 4.5
Analysis Note
The activity was determined by the dose dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Storage Class
8A - Combustible corrosive hazardous materials
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Interplay between TGF-? signaling and receptor tyrosine kinases in tumor development.
Shi Q and Chen YG
Science China: Life Sciences, 60(10), 1133-1114 (2017)
PDGFRB Promotes Liver Metastasis Formation of Mesenchymal-Like Colorectal Tumor Cells
Steller EJA, et al.
Neoplasia, 15, 204?217 - 204?217 (2013)
Measurement of in-plane elasticity of live cell layers using a pressure sensor embedded microfluidic device
Lin CH, et al.
Scientific Reports, 1-14 (2016)
Huiyuan Zheng et al.
Carcinogenesis, 40(4), 580-591 (2018-11-13)
Transforming growth factor beta (TGFβ) is multifunctional cytokine that is involved in the coordination and regulation of many cellular homeostatic processes. Compromised TGFβ activity has been attributed to promotion of human cancers. Recent studies have identified a role for TGFβ
Bin Li et al.
American journal of translational research, 14(4), 2199-2211 (2022-05-14)
Hepatitis B virus (HBV) infection is the main reason for liver cirrhosis. The purpose of this research was to probe into the role and underlying mechanism of circ_myotubularin 1 (circ_MTM1) in HBV-related liver fibrosis (LF). HBV surface antigen (HBsAg) and
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