This material is sold based on Sigma Units. One Sigma Unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.
H8891
Heparinase III from Flavobacterium heparinum
Lyophilized powder stabilized with approx. 25% (w/w) bovine serum albumin, ≥30 units/mg protein (enzyme + BSA)
Synonym(s):
Heparin Lyase III, Heparitinase I, Heparitinase from Flavobacterium heparinum
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5 UNITS
$288.00
10 UNITS
$496.00
50 UNITS
$1,970.00
$288.00
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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biological source
bacterial (Flavobacterium heparinum)
Quality Level
conjugate
conjugate (Glucosaminoglycan)
form
lyophilized powder
specific activity
≥30 units/mg protein (enzyme + BSA)
mol wt
70.8 kDa
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
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This Item | H2519 | H6512 | H3917 |
|---|---|---|---|
| specific activity ≥30 units/mg protein (enzyme + BSA) | specific activity ≥200 units/mg protein (enzyme + BSA) | specific activity ≥100 units/mg protein (enzyme + BSA) | specific activity ≥200 units/mg protein |
| biological source bacterial (Flavobacterium heparinum) | biological source - | biological source bacterial (Flavobacterium heparinum) | biological source bacterial (Flavobacterium heparinum) |
| form lyophilized powder | form lyophilized solid | form lyophilized powder | form lyophilized powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| mol wt 70.8 kDa | mol wt 42.8 kDa | mol wt 84.1 kDa | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Application
Biochem/physiol Actions
Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate.
Heparinase III prevents the formation of new blood vessels in vivo.[4] It specifically degrades heparin sulphate at the HNY-I and HNY,6X-G2 links. Unlike other heparinise, heparinase III does not contain the amino acid cysteine. It contains 13 histidine residues, which is responsible for its catalytic action.[5] This enzyme is not active towards heparin or low molecular weight heparins.
Other Notes
One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units. Package sizes are sold in Sigma units.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Fluid shear stress primes mouse embryonic stem cells for differentiation in a self-renewing environment via heparan sulfate proteoglycans transduction
Toh YC and Voldman J
Faseb Journal, 25(4), 1208-1217 (2011)
FGF binding by extracellular matrix components of Wharton's jelly
Malkowski A, et al.
Acta Biochimica Polonica, 54(2), 357-357 (2007)
Erin M Smith et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(51), 21683-21688 (2009-12-08)
Platelet-derived growth factor (PDGF) signaling is essential for processes involving cell motility and differentiation during embryonic development in a wide variety of organisms including the mouse, frog, zebrafish, and sea urchin. In early Xenopus laevis embryos, PDGF-AA provides guidance cues
Md Nasimuzzaman et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 20(6), 1158-1166 (2012-03-22)
Foamy viruses (FVs) (spumaretroviruses) are good alternative to retroviruses as gene therapy vector. Despite four decades since the discovery of FV, its receptor molecule is still unknown. FV vector transduction of human CD34(+) cells was inhibited by culture with fibronectin.
Histidine 295 and histidine 510 are crucial for the enzymatic degradation of heparan sulfate by heparinase III
Pojasek K, et al.
Biochemistry, 39(14), 4012-4019 (2000)
Articles
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
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Is Product No. H8891, Heparinase III sold based on Sigma Units or International Units?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Does Product No. H8891, Heparinase III, contain calcium?
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This product is lyophilized from a solution which is 75 mM NaCl/ 5mM NaH2PO4. We have no reason to believe that calcium ions are present.
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