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H9250

Sigma-Aldrich

Histone from calf thymus

Type II-A, lyophilized powder

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Synonym(s):
histone type VIII-S
CAS Number:
9064-47-5
Beilstein:
1895465
MDL number:
MFCD00131329
NACRES:
NA.51

biological source

bovine (calf) thymus

type

Type II-A

form

lyophilized powder

application(s)

cell analysis

storage temp.

2-8°C

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This Item
H5505H4380H2042
Histone from calf thymus Type II-A, lyophilized powder

Sigma-Aldrich

H9250

Histone from calf thymus

Histone from calf thymus Type III-S

Sigma-Aldrich

H5505

Histone from calf thymus

Histone from calf thymus Type VIII-S

Sigma-Aldrich

H4380

Histone from calf thymus

Sigma-Aldrich

Sigma-Aldrich

H2042

Histone H2A human

form

lyophilized powder

form

powder

form

powder

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

type

Type II-A

type

Type III-S

type

Type VIII-S

type

-

application(s)

cell analysis

application(s)

-

application(s)

-

application(s)

cell analysis

General description

Histones are octameric proteins containing H3, H4, H2A and H2B. It is the core protein around which DNA is wound forming the nucleosome structure. The core histone proteins contain globular domains. Each protein is composed of basic amino acids at its N-terminal. The H2A has an extra amino acid at its C-terminal end forming a histone tail. The nucleosome structure is stabilized by the linker histones during chromatin condensation.

Application

Histone from calf thymus has been used:
  • in methyltransferase reaction mixture for methyltransferase assay
  • in in vitro binding assay, to explore the potential binding of the MORF4 (mortality factor on chromosome 4) - related gene on chromosome 15 (MRG15) chromo domain with histone
  • in in vitro coculture assays, to stimulate liver resident Kupffer cells

Biochem/physiol Actions

The core histones are involved in histone-histone interactions and maintains the organization of the nucleosomal DNA. The histone tail helps in forming higher-order nucleosomal structures. The overall structure and action of chromatin fiber is regulated by histone modifications.

Features and Benefits

Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.

Preparation Note

Unfractionated whole histone

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Histones and histone modifications
Peterson CL, et al.
Current Biology, 14(14), R546-R551 (2004)
Yannick Tauran et al.
Scientific reports, 9(1), 5816-5816 (2019-04-11)
By means of Silicon Nano Tweezers (SNTs) the effects on the mechanical properties of λ-phage DNA during interaction with calf thymus nucleosome to form an artificial chromatin analog were measured. At a concentration of 100 nM, a nucleosome solution induced a
Adam F Kebede et al.
Nature structural & molecular biology, 24(12), 1048-1056 (2017-10-24)
Histones are highly covalently modified, but the functions of many of these modifications remain unknown. In particular, it is unclear how histone marks are coupled to cellular metabolism and how this coupling affects chromatin architecture. We identified histone H3 Lys14
Juan-Carlos Cabrera et al.
Glycobiology, 20(6), 775-786 (2010-03-05)
Plant cell walls undergo remodeling during growth and development and are the first target of many invading pathogens. Acidic pectin (homogalacturonans) binds calcium and forms chain dimers called egg boxes and even multimers at higher calcium ion concentrations. Chitosan, the
Histones activate the NLRP3 inflammasome in Kupffer cells during sterile inflammatory liver injury
Huang H, et al.
Journal of Immunology, 191(5), 2665-2679 (2013)
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