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MilliporeSigma

H9394

Hanks′ Balanced Salt solution

Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

HBSS

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100 ML

$16.30

500 ML

$31.80

1 L

$57.30

6 X 500 ML

$168.00

6 X 1 L

$310.00

24 X 500 ML

$604.00

$16.30

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

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sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L

shipped in

ambient

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This Item
H664855021CH8264
Hanks’ Balanced Salt solution Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

H9394

Hanks′ Balanced Salt solution

Sigma-Aldrich

Sigma-Aldrich

H6648

Hanks′ Balanced Salt solution

Hanks’ Balanced Salt solution HBSS Modified, with phenol red, without calcium, without magnesium, liquid

SAFC

55021C

Hanks′ Balanced Salt solution

Sigma-Aldrich

Sigma-Aldrich

H8264

Hanks′ Balanced Salt solution

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile

sterility

sterile-filtered

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: no

components

NaHCO3: 350 mg/L
phenol red: 10.62 mg/L
Hanks’ salts (2% CO2): yes

components

phenol red: no
glucose: 1.0 g/L (Dextro)
NaHCO3: 0.35 g/L

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

General description

Hanks′ Balanced Salt solution is recommended for suspension cultures or whenever cell clumping is a problem.

Application

Hanks′ Balanced Salt solution has been used:
  • as a component of the culture medium for culturing of mouse cortical slices[1]
  • as a component of the dissociation buffer for incubation of human-induced pluripotent stem cells (hiPSCs) and hiPSC-derived cardiomyocytes (CMs)[2]
  • for in situ perfusions of mice liver[3]

Biochem/physiol Actions

Balanced salt solution in cell culture plays multiple roles such as, an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance, and provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It is also involved in providing a buffering system to maintain the medium within the physiological pH range (7.2-7.6) and provides the principal energy source for cell metabolism.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBM0561
RNBL8854
RNBL5851
RNBL4924
RNBL4477

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Norma Jäppinen et al.
Nature communications, 10(1), 281-281 (2019-01-19)
Macrophages serve multiple functions including immune regulation, morphogenesis, tissue homeostasis and healing reactions. The current paradigm holds that mammary gland macrophages first arise postnatally during the prepubertal period from the bone marrow-derived monocytes. Here we delineate the origins of tissue-resident
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Human gene therapy, 31(9-10), 575-589 (2020-02-01)
Adeno-associated virus (AAV) vectors are quickly becoming the vectors of choice for therapeutic gene delivery. To date, hundreds of natural isolates and bioengineered variants have been reported. While factors such as high production titer and low immunoreactivity are important to
Sunny Nigam et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 36(3), 670-684 (2016-01-23)
The performance of complex networks, like the brain, depends on how effectively their elements communicate. Despite the importance of communication, it is virtually unknown how information is transferred in local cortical networks, consisting of hundreds of closely spaced neurons. To
Pratyusha Mandal et al.
Viruses, 12(11) (2020-11-01)
Cytomegaloviruses all encode the viral inhibitor of caspase-8-induced apoptosis (vICA). After binding to this initiator caspase, vICA blocks caspase-8 proteolytic activity and ability to activate caspase-3 and/or caspase-7. In this manner, vICA has long been known to prevent apoptosis triggered
Margret Bessadottir et al.
PloS one, 7(12), e51296-e51296 (2012-12-12)
The lichen compound usnic acid (UA) is a lipophilic weak acid that acts as a proton shuttle and causes loss of mitochondrial inner membrane potential. In the current study we show that UA treatment induced the formation of autophagosomes in
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Protocols

Trypsin Cell Dissociation Protocol

Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.

StableCell™ Trypsin Solutions

StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.

Questions

  1. After opening the container of H9394-500mL solution, how long is the product good for before requiring to discard?

    1 answer

    1. The minimum shelf life exclusively refers to originally sealed packages stored under adequate conditions as shown on the label, on the technical data sheet or indicated on our homepage.

      The shelf life after opening is depending on various factors (e.g. storage temperatures, humidity, atmosphere, etc.) which might affect the stability after opening. Influencing outside factors can not be considered by the company .

      Ideally, if the product is handled according to good laboratory practices and stored under the prescribed conditions, the shelf life after opening tends to coincide with the expiry date of the unopened package. However, it remains the user's responsibility to define the stability criteria of the product after opening/during use.

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