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H9395

Sigma-Aldrich

α-Hemolysin from Staphylococcus aureus

lyophilized powder, Protein ~60 % by Lowry, ≥10,000 units/mg protein

All Photos(2)
Synonym(s):
α-Toxin
CAS Number:
94716-94-6
MDL number:
MFCD00130489
NACRES:
NA.56

biological source

Staphylococcus aureus

Quality Level

300

form

lyophilized powder

specific activity

≥10,000 units/mg protein

contains

sodium citrate buffer as balance

composition

Protein, ~60% Lowry

solubility

H2O: soluble 0.49-0.51 mg/mL

UniProt accession no.

Q2G1X0

storage temp.

2-8°C

Gene Information

Staphylococcus aureus ... SAOUHSC_01121(3920722)

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P6031L7386P3391
α-Hemolysin from Staphylococcus aureus lyophilized powder, Protein ~60 % by Lowry, ≥10,000 units/mg protein

Sigma-Aldrich

H9395

α-Hemolysin from Staphylococcus aureus

Protein A from Staphylococcus aureus Soluble, essentially salt-free, lyophilized powder, extracellular

Sigma-Aldrich

P6031

Protein A from Staphylococcus aureus

Lysostaphin from Staphylococcus staphylolyticus lyophilized powder, Protein 50-70 % by biuret, ≥500 units/mg protein

Sigma-Aldrich

L7386

Lysostaphin from Staphylococcus staphylolyticus

Millipore

Millipore

P3391

Protein A-Sepharose® from Staphylococcus aureus

biological source

Staphylococcus aureus

biological source

Staphylococcus aureus

biological source

microbial (Staphylococcus staphylotyticus)

biological source

-

form

lyophilized powder

form

essentially salt-free, lyophilized powder

form

lyophilized powder

form

lyophilized powder

specific activity

≥10,000 units/mg protein

specific activity

-

specific activity

≥500 units/mg protein

specific activity

-

solubility

H2O: soluble 0.49-0.51 mg/mL

solubility

H2O: soluble 1 mg/mL, clear, colorless

solubility

water: 2.00-2.10 mg/mL, clear to slightly hazy, colorless to light yellow

solubility

water: 1 g/L at 20 °C

UniProt accession no.

Q2G1X0

UniProt accession no.

P02976

UniProt accession no.

-

UniProt accession no.

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

General description

α-Hemolysin, a pore-forming cytotoxin is an extracellular protein secreted by most strains of pathogenic Staphylococcus aureus. It is secreted as a water-soluble monomer and is a small β-barrel protein.

Application

α-Hemolysin from Staphylococcus aureus has been used:
  • as a component of electrolyte solution for testing pore formation in lipid bilayer using electrophysiological measurements
  • to test its osteogenesis suppressive effects in bone marrow stromal cells (BMSCs)
  • in the preparation of α-hemolysin molecular imprinted polymer (MIP) for Biacore and surface plasmon resonance

α-Hemolysin was used in a study to test the efflux pump and haemolysin activity of Escherichia coli of dairy origin. It was also used to test its adaptation to benzalkonium chloride and the effect of ciprofloxacin on biofilm formation.

Biochem/physiol Actions

α-Hemolysin is selectively hemolytic and the monomeric form binds to a membrane and specific receptors are not required for binding. Upon binding to biological membranes and/or artificial membranes, self-oligomerization occurs, resulting in ring structures (hexameric aggregates) believed to represent transmembrane pores, which are permeable to ions and small metabolites. It has a marked preference for rabbit red blood cells. α-hemolysin stimulates cellular phospholipases and induces a Ca2+ influx. It leads to membrane disruption of the endothelial barrier and leakage of cytoplasmic components and osmotic lysis of the cells. α-hemolysin is implicated in the pathogenesis of sepsis.

Packaging

Package size based on protein content

Unit Definition

One hemolytic unit will cause 50% lysis of a 1% suspension of rabbit red blood cells in phosphate buffered saline, pH 7.0, containing 1% bovine serum albumin after 30 min at 37 °C followed by refrigeration for 30 min at 4 °C.

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Warning

Hazard Statements

H315 - H319 - H371

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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