The compound should be dissolved initially at 50mg/mL in a 2:1 mixture of Formic acid and water, respectively. Once dissolved, the stock can be diluted in a buffer or culture medium. This is not a sterile product. Stock solutions should be filter sterilized prior to adding to sterile medium.
H9636
Hypoxanthine
powder, suitable for cell culture, BioReagent
Synonym(s):
6-Hydroxypurine
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About This Item
Empirical Formula (Hill Notation):
C5H4N4O
CAS Number:
Molecular Weight:
136.11
NACRES:
NA.75
PubChem Substance ID:
UNSPSC Code:
12352207
EC Number:
200-697-3
MDL number:
Beilstein/REAXYS Number:
5811
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Product Name
Hypoxanthine, powder, BioReagent, suitable for cell culture
InChI key
FDGQSTZJBFJUBT-UHFFFAOYSA-N
InChI
1S/C5H4N4O/c10-5-3-4(7-1-6-3)8-2-9-5/h1-2H,(H2,6,7,8,9,10)
SMILES string
O=C1NC=Nc2nc[nH]c12
product line
BioReagent
assay
≥99%
form
powder
technique(s)
cell culture | mammalian: suitable
mp
>300 °C (lit.)
solubility
formic acid: water (2:1): 50 mg/mL
Quality Level
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1 of 4
This Item | Y0000441 | Y0002147 | H9377 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) - |
| product line BioReagent | product line - | product line - | product line - |
| Quality Level 200 | Quality Level - | Quality Level - | Quality Level 300 |
| form powder | form - | form - | form powder |
| assay ≥99% | assay - | assay - | assay ≥99.0% |
| solubility formic acid: water (2:1): 50 mg/mL | solubility - | solubility - | solubility formic acid: water (2:1): 50 mg/mL, clear to slightly hazy, colorless to yellow |
Application
Hypoxanthine is a nutrient additive for a variety of cell culture applications involving bacterial, parasite (Plasmodium falciparum) and animal cells. Hypoxanthine is a component of selection media used in hybridoma technologies.
Hypoxanthine has been used:
- as a supplement in HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) -buffered Roswell park memorial institute (RPMI) to culture Plasmodium falciparum strain IT/FCR3[1]
- in the preparation of Dulbecco′s modified eagle′s medium (DMEM) + MXH for the selection of chimeric modified vaccinia virus ankara (MVA)[2]
- to generate superoxide anions[3]
- to prepare hybridoma selection and maintenance media.
Biochem/physiol Actions
Hypoxanthine is capable of stimulating cell death. It can also induce reactive oxygen species (ROS). It results in endothelial dysfunction via apoptosis, stimulated by oxidative stress.[4]
General description
Hypoxanthine (6-hydroxypurine), a purine derivative is a naturally occurring compound. It is the deaminated form of adenine and a breakdown product of adenosine monophosphate (AMP).[5]
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Biochemical Methods of Estimating the Time Since Death
Human Body Decomposition, 53-90 (2016)
Construction of FWPV Chimaeric MVA
Buttigieg KR, et al.
Bio-protocol, 5(1), 1-11 (2019)
Hypoxanthine causes endothelial dysfunction through oxidative stress-induced apoptosis
Kim YJ, et al.
Biochemical and biophysical research communications, 482(4), 821-827 (2017)
Binding of Plasmodium falciparum to CD36 can be shielded by the glycocalyx
Hempel C, et al.
Malaria Journal, 16(1), 1-11 (2017)
Diana Tamayo et al.
PLoS neglected tropical diseases, 10(3), e0004481-e0004481 (2016-03-11)
The ability of Paracoccidioides to defend itself against reactive oxygen species (ROS) produced by host effector cells is a prerequisite to survive. To counteract these radicals, Paracoccidioides expresses, among different antioxidant enzymes, superoxide dismutases (SODs). In this study, we identified
Articles
Uric acid present in animal sera impacts serum-supplemented classical cell culture systems, influencing cell culture performance.
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What is the solubility of HYPOXANTHINE in water and How to dissolve HYPOXANTHINE in water for cell culture.
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