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H9636

Sigma-Aldrich

Hypoxanthine

powder, BioReagent, suitable for cell culture

Synonym(s):
6-Hydroxypurine
Empirical Formula (Hill Notation):
C5H4N4O
CAS Number:
Molecular Weight:
136.11
Beilstein:
5811
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

product line

BioReagent

Quality Level

Assay

≥99%

form

powder

technique(s)

cell culture | mammalian: suitable

mp

>300 °C (lit.)

solubility

formic acid: water (2:1): 50 mg/mL

SMILES string

O=C1NC=Nc2nc[nH]c12

InChI

1S/C5H4N4O/c10-5-3-4(7-1-6-3)8-2-9-5/h1-2H,(H2,6,7,8,9,10)

InChI key

FDGQSTZJBFJUBT-UHFFFAOYSA-N

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This Item
4010CBCY0000441Y0002147
Hypoxanthine powder, BioReagent, suitable for cell culture

Sigma-Aldrich

H9636

Hypoxanthine

Hypoxanthine Xanthine oxidase substrate.

Millipore

4010CBC

Hypoxanthine

Didanosine impurity A, European Pharmacopoeia (EP) Reference Standard

Y0000441

Didanosine impurity A,

Mercaptopurine impurity A CRS, European Pharmacopoeia (EP) Reference Standard

Y0002147

Mercaptopurine impurity A

assay

≥99%

assay

≥99% (HPLC)

assay

-

assay

-

form

powder

form

solid

form

-

form

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

mp

>300 °C (lit.)

mp

-

mp

>300 °C (lit.)

mp

>300 °C (lit.)

solubility

formic acid: water (2:1): 50 mg/mL

solubility

1 M HCl: 20 mg/mL, clear (faint color solution), DMF: 5 mg/mL, cloudy (with particulates), DMSO: 5 mg/mL, clear, colorless (with few particulates), hydrogen: 5 mg/mL, cloudy (with particulates)

solubility

-

solubility

-

General description

Hypoxanthine (6-hydroxypurine), a purine derivative is a naturally occurring compound. It is the deaminated form of adenine and a breakdown product of adenosine monophosphate (AMP).

Application

Hypoxanthine is a nutrient additive for a variety of cell culture applications involving bacterial, parasite (Plasmodium falciparum) and animal cells. Hypoxanthine is a component of selection media used in hybridoma technologies.
Hypoxanthine has been used:
  • as a supplement in HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) -buffered Roswell park memorial institute (RPMI) to culture Plasmodium falciparum strain IT/FCR3
  • in the preparation of Dulbecco′s modified eagle′s medium (DMEM) + MXH for the selection of chimeric modified vaccinia virus ankara (MVA)
  • to generate superoxide anions
  • to prepare hybridoma selection and maintenance media.

Biochem/physiol Actions

Hypoxanthine is capable of stimulating cell death. It can also induce reactive oxygen species (ROS). It results in endothelial dysfunction via apoptosis, stimulated by oxidative stress.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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Hypoxanthine causes endothelial dysfunction through oxidative stress-induced apoptosis
Kim YJ, et al.
Biochemical and biophysical research communications, 482(4), 821-827 (2017)
Construction of FWPV Chimaeric MVA
Buttigieg KR, et al.
Bio-protocol, 5(1), 1-11 (2019)
Biochemical Methods of Estimating the Time Since Death
Human Body Decomposition, 53-90 (2016)
Binding of Plasmodium falciparum to CD36 can be shielded by the glycocalyx
Hempel C, et al.
Malaria Journal, 16(1), 1-11 (2017)
Diana Tamayo et al.
PLoS neglected tropical diseases, 10(3), e0004481-e0004481 (2016-03-11)
The ability of Paracoccidioides to defend itself against reactive oxygen species (ROS) produced by host effector cells is a prerequisite to survive. To counteract these radicals, Paracoccidioides expresses, among different antioxidant enzymes, superoxide dismutases (SODs). In this study, we identified

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