H9886
p-Hydroxybenzoate Hydroxylase from Pseudomonas sp.
lyophilized powder, ~20 units/mg solid
Synonym(s):
4-Hydroxybenzoate, NADPH:oxygen oxidoreductase (3-hydroxylating)
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
form
lyophilized powder
specific activity
~20 units/mg solid
mol wt
55-~60 kDa
color
yellow
storage temp.
−20°C
Application
This enzyme is useful for enzymatic determination of choline esterase when coupled with protocatechuate 3, 4-dioxygenase.
Physical properties
Michaelis constants : 2.0 x 10-5M (p-Hydroxybenzoate), 4.0 x 10-5M (NADPH)
Structure : One mol of FAD per mol of enzyme
Inhibitors : Ag+, Hg++, PCMB, SDS
Optimum pH : 7.7 - 7.9
Optimum temperature : 35oC
pH Stability : pH 5.0 - 7.5 (25oC, 72hr)
Thermal stability : below 40oC (pH 6.0, 15min)
Structure : One mol of FAD per mol of enzyme
Inhibitors : Ag+, Hg++, PCMB, SDS
Optimum pH : 7.7 - 7.9
Optimum temperature : 35oC
pH Stability : pH 5.0 - 7.5 (25oC, 72hr)
Thermal stability : below 40oC (pH 6.0, 15min)
Unit Definition
One unit will hydroxylate 1.0 μmole of p-hydroxybenzoate to protocatechuate per min at pH 8.2 at 37 °C in the presence of NADPH.
Physical form
Contains mannitol and stabilizers
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Lindsay J Cole et al.
Biochemistry, 44(22), 8047-8058 (2005-06-01)
p-Hydroxybenzoate hydroxylase (PHBH) is a homodimeric flavoprotein monooxygenase that catalyzes the hydroxylation of p-hydroxybenzoate to form 3,4-dihydroxybenzoate. Controlled catalysis is achieved by movement of the flavin and protein between three conformations, in, out, and open [Entsch, B., et al. (2005)
Adrie H Westphal et al.
The Journal of biological chemistry, 281(16), 11074-11081 (2006-02-24)
We have used the flavoenzyme p-hydroxybenzoate hydroxylase (PHBH) to illustrate that a strongly fluorescent donor label can communicate with the flavin via single-pair Förster resonance energy transfer (spFRET). The accessible Cys-116 of PHBH was labeled with two different fluorescent maleimides
Robert D Bach et al.
Journal of the American Chemical Society, 126(1), 127-142 (2004-01-08)
A model C-(4a)-flavinhydroperoxide (FlHOOH) is described that contains the tricyclic isoalloxazine moiety, the C-4a-hydroperoxide functionality, and a beta-hydroxyethyl group to model the effect of the 2'-OH group of the ribityl side chain of native FADHOOH. The electronic structures of this
Yue Cui et al.
Biosensors & bioelectronics, 22(11), 2754-2758 (2006-12-02)
This work reports the development of an amperometric glucose-6-phosphate biosensor by coimmobilizing p-hydroxybenzoate hydroxylase (HBH) and glucose-6-phosphate dehydrogenase (G6PDH) on a screen-printed electrode. The principle of the determination scheme is as follows: G6PDH catalyzes the specific dehydrogenation of glucose-6-phosphate by
Mariliz Ortiz-Maldonado et al.
Biochemistry, 43(6), 1569-1579 (2004-02-11)
Para-hydroxybenzoate hydroxylase is a flavoprotein monooxygenase that catalyzes a reaction in two parts: reduction of the enzyme cofactor, FAD, by NADPH in response to binding p-hydroxybenzoate to the enzyme, and oxidation of reduced FAD with oxygen to form a hydroperoxide
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