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HIS-Select® Nickel Magnetic Agarose Beads

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Synonym(s):
nickel charged magnetic beaded agarose
NACRES:
NA.56

conjugate

magnetic beads

Quality Level

form

suspension

shelf life

2 yr (Unopened product)

matrix

6% beaded magnetic agarose

capacity

≥15 mg/mL binding capacity

storage temp.

2-8°C

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This Item
H05377066616-662
Millipore

Millipore

70666

Ni-NTA His•Bind® Resin

form

suspension

form

suspension

form

slurry

form

-

shelf life

2 yr (Unopened product)

shelf life

-

shelf life

-

shelf life

-

matrix

6% beaded magnetic agarose

matrix

Highly cross-linked 6% Beaded Agarose

matrix

-

matrix

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

400

Quality Level

100

General description

HIS-Select Magnetic Agarose Beads consist of paramagnetic, immobilized metal-ion affinity chromatography (IMAC) resin that contain a proprietary quadridentate chelate, which is bound with nickel and covalently attached through a non-charged, hydrophilic linker to magnetic beaded agarose. The magnetic properties of the beads aid in manipulations, such as repetitive washings, and recovery of the protein bound beads. This leads to greater experimental reproducibility and more accurate quantitation of the His-tagged proteins of interest.

Application

The HIS-Select Nickel Magnetic Agarose Beads are designed for use in automated and small-scale affinity capture (molecular pull-down) purifications of histidine-tagged protein / His-tag protein while exhibiting low non-specific binding of other proteins. The beads can be used to purify His-tagged proteins under native and denaturing conditions.

Physical form

Supplied as a 50% slurry suspension in 30% Ethanol.

Legal Information

HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC

related product

Product No.
Description
Pricing

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 3 - Carc. 1B - Flam. Liq. 3 - Repr. 1B - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

86.0 °F

Flash Point(C)

30 °C


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