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MilliporeSigma

H9914

HIS-Select® Nickel Magnetic Agarose Beads

Synonym(s):

nickel charged magnetic beaded agarose

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$192.00

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$764.00

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About This Item

NACRES:
NA.56
UNSPSC Code:
41106500
Form:
suspension

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conjugate

magnetic beads

form

suspension

shelf life

2 yr (Unopened product)

matrix

6% beaded magnetic agarose

capacity

≥15 mg/mL binding capacity

storage temp.

2-8°C

Quality Level

200

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This Item
G0924SAE0201LSKMAGH
HIS-Select® Nickel Magnetic Agarose Beads

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H9914

HIS-Select® Nickel Magnetic Agarose Beads

Glutathione High Capacity Magnetic Agarose Beads (1:1 suspension in a 30% ethanol solution)

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G0924

Glutathione High Capacity Magnetic Agarose Beads

Anti-C-Myc Magnetic Beads Magnetic agarose, suspension

Sigma-Aldrich

SAE0201

Anti-C-Myc Magnetic Beads

PureProteome Nickel Magnetic Bead System PureProteome Nickel Magnetic Beads provide researchers with a powerful bead system to help purify polyhistidine-tagged recombinant proteins

Millipore

LSKMAGH

PureProteome Nickel Magnetic Bead System

form

suspension

form

(1:1 suspension in a 30% ethanol solution)

form

suspension

form

liquid

shelf life

2 yr (Unopened product)

shelf life

-

shelf life

-

shelf life

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

conjugate

magnetic beads

conjugate

magnetic beads

conjugate

magnetic beads conjugate

conjugate

magnetic beads conjugate

matrix

6% beaded magnetic agarose

matrix

4% beaded magnetic agarose

matrix

-

matrix

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

General description

HIS-Select Magnetic Agarose Beads consist of paramagnetic, immobilized metal-ion affinity chromatography (IMAC) resin that contain a proprietary quadridentate chelate, which is bound with nickel and covalently attached through a non-charged, hydrophilic linker to magnetic beaded agarose. The magnetic properties of the beads aid in manipulations, such as repetitive washings, and recovery of the protein bound beads. This leads to greater experimental reproducibility and more accurate quantitation of the His-tagged proteins of interest.

Application

The HIS-Select Nickel Magnetic Agarose Beads are designed for use in automated and small-scale affinity capture (molecular pull-down) purifications of histidine-tagged protein / His-tag protein while exhibiting low non-specific binding of other proteins. The beads can be used to purify His-tagged proteins under native and denaturing conditions.

Physical form

Supplied as a 50% slurry suspension in 30% Ethanol.

Legal Information

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

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Product No.
Description
Pricing

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Carc. 1B - Flam. Liq. 3 - Repr. 1B - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

3 - Flammable liquids

wgk

WGK 3

flash_point_f

86.0 °F

flash_point_c

30 °C

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Certificates of Analysis (COA)

Lot/Batch Number
0000489809
0000489807
0000489807
0000489809
0000464376

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Sonja Hänzelmann et al.
Clinical epigenetics, 7, 19-19 (2015-03-13)
Primary cells enter replicative senescence after a limited number of cell divisions. This process needs to be considered in cell culture experiments, and it is particularly important for regenerative medicine. Replicative senescence is associated with reproducible changes in DNA methylation
Mei Suen Kong et al.
Science signaling, 12(567) (2019-02-07)
T cell activation is initiated by signaling molecules downstream of the T cell receptor (TCR) that are organized by adaptor proteins. CIN85 (Cbl-interacting protein of 85 kDa) is one such adaptor protein. Here, we showed that CIN85 limited T cell
Yun Wei et al.
Journal of materials chemistry. B, 1(15), 2066-2071 (2013-04-21)
The imidazolium cation was chosen as protein selective affinity group and a kind of ionic liquid magnetic microspheres was developed by immobilizing imidazolium cations onto the surface of silica-coated magnetic microspheres to form imidazolium ionic liquid modified magnetic microspheres (Fe3O4@SiO2@IL).
Joshua W Burgess et al.
American journal of respiratory cell and molecular biology, 41(6), 714-721 (2009-03-17)
Pneumocystis organisms are opportunistic fungal pathogens that cause significant pneumonia in immune-compromised hosts. Recent evidence has suggested that Pneumocystis carinii exists as separate mating types, and expresses and regulates proteins that govern meiosis and progression of the life cycle. This
Philip Vitorino et al.
Nature, 519(7544), 425-430 (2015-03-25)
Cell migration is a stepwise process that coordinates multiple molecular machineries. Using in vitro angiogenesis screens with short interfering RNA and chemical inhibitors, we define here a MAP4K4-moesin-talin-β1-integrin molecular pathway that promotes efficient plasma membrane retraction during endothelial cell migration.
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