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HCLROS1

Sigma-Aldrich

ROS1 Analyte Control

Cell line controls for immunohistochemistry and in situ hybridization

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NACRES:
NA.47

biological source

human cell culture

packaging

pkg of 1 ea cell microarray block (HCL024)
pkg of 2 slides (HCL022)
pkg of 5 slides (HCL023)

manufacturer/tradename

Histocyte

storage temp.

2-8°C

General description

The ROS1 Analyte Control has two cell line cores which demonstrate positive and negative expression of ROS1.
HistoCyte Laboratories control slides and blocks are made from cultured human cell lines to provide the required range of expression of a specific biomarker. The cell lines are processed using proprietary methods that allow for the creation of a high density embedded cell block with cores that retain their cellular morphology. Available as pre-cut unstained slides or as cell microarray blocks for sectioning.

Application

Ideal for use as a same slide control for immunohistochemistry (IHC) and in situ hybridization (ISH).
The ROS1 Analyte Control histology slides have two cell line cores providing positive and negative expression of ROS1; one cell line contains an SLC34A2-ROS translocation. Analyte controls are placed at one end of slide, allowing sample application on same slide for valid comparison.
The ROS1 Analyte Control cell microarray block contains two cell line cores that are 2 mm in diameter and between 3.0-3.5 mm in length. The cell microarray block may yield over 300 sections depending on thickness.

Preparation Note

Cell microarray blocks are fixed in 10% neutral buffered formalin and embedded in paraffin wax. Pre-cut unstained slides are prepared from 4 μm thick sections, mounted on positively charged slides, and dried at 37 °C overnight.

Other Notes

For certificates of analysis, contact info@histocyte.com.
ROS1 Analyte Control brochure is available from HistoCyte Laboratories.

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