HPA005785

Sigma-Aldrich

Anti-CD44 antibody produced in rabbit

enhanced validation

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):
Cd44 Antibody, Anti-Hermes antigen antibody produced in rabbit, Anti-Epican antibody produced in rabbit, Anti-CDw44 antibody produced in rabbit, Anti-GP90 lymphocyte homing/adhesion receptor antibody produced in rabbit, Anti-CD44 antigen precursor antibody produced in rabbit, Anti-Extracellular matrix receptor-III antibody produced in rabbit, CD44 Antibody - Anti-CD44 antibody produced in rabbit, Anti-HUTCH-I antibody produced in rabbit, Anti-Heparan sulfate proteoglycan antibody produced in rabbit, Anti-Hyaluronate receptor antibody produced in rabbit, Anti-PGP-1 antibody produced in rabbit, Anti-Phagocytic glycoprotein I antibody produced in rabbit
MDL number:
Human Protein Atlas Number:

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

packaging

antibody small pack of 25 μL

enhanced validation

orthogonal RNAseq
Learn more about Antibody Enhanced Validation

application(s)

immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:1000-1:2500

immunogen sequence

DDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIPATTLMSTSATATETATKRQETWDWFSWLFLPSESKNHLHTTTQMAGTSSNTISAGWEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPR

conjugate

unconjugated

Featured Industry

Research Pathology

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CD44(960)

General description

The CD44 (cell-surface glycoprotein) gene encodes a transmembrane glycoprotein and has 20 exons. It is mapped to human chromosome 11p13.

Immunogen

CD44 antigen precursor recombinant protein epitope signature tag (PrEST)

Application

Anti-CD44 antibody produced in rabbit has been used:
  • in immunohistochemistry
  • in western blotting
  • in immunofluorescence staining to characterize the immunophenotype of the mammospheres

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

Biochem/physiol Actions

Overexpression of CD44 (cell-surface glycoprotein) leads to esophageal squamous cell carcinoma (ESCC). It is involved in cell-cell and cell-extracellular matrix interactions. It plays an important role in lymphocyte homing and lymphocyte activation.
CD44 is a hyaluronic acid receptor that regulates cell repair, adhesion and inflammation. This protein has been implicated in cancer aggressiveness, metastasis and growth. Thus, CD44 is used as a cell-surface marker for several cancers . Anti-CD44 antibody is specific for CD44 in humans.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST83079.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Ran Ma et al.
Laboratory investigation; a journal of technical methods and pathology, 94(7), 796-805 (2014-04-30)
Breast cancer is a unique tumor disease in terms of the stringent requirement of predictive biomarker assessments. As recommended by current international guidelines, the established markers consist of estrogen receptor (ER), progesterone receptor, human epidermal growth factor and Ki67, and...
Jung-Hyun Rho et al.
Gut, 67(3), 473-484 (2016-11-09)
To discover and confirm blood-based colon cancer early-detection markers. We created a high-density antibody microarray to detect differences in protein levels in plasma from individuals diagnosed with colon cancer <3 years after blood was drawn (ie, prediagnostic) and cancer-free, matched controls....
Vincent Petit et al.
Laboratory investigation; a journal of technical methods and pathology, 93(5), 611-621 (2013-03-06)
Metabolic adaptations and changes in the expression of nutrient transporters are known to accompany tumorigenic processes. Nevertheless, in the context of solid tumors, studies of metabolism are hindered by a paucity of tools allowing the identification of cell surface transporters...
Autophagy supports generation of cells with high CD44 expression via modulation of oxidative stress and Parkin-mediated mitochondrial clearance
Whelan KA, et al.
Oncogene, 36(34), 4843-4843 (2017)
Significance of CD44 and CD24 as cancer stem cell markers: an enduring ambiguity.
Jaggupilli, A., and Elkord, E.
Clinical & Developmental Immunology, doi: 10-doi: 10 (2012)
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