HPA011226

Sigma-Aldrich

Anti-PTGER4 antibody produced in rabbit

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):
Anti-Prostanoid EP4 receptor, Anti-Prostaglandin E2 receptor EP4 subtype, Anti-PGE receptor, EP4 subtype
Human Protein Atlas Number:

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

packaging

antibody small pack of 25 μL

application(s)

immunohistochemistry: 1:20- 1:50

immunogen sequence

QLYQPSLEREVSKNPDLQAIRIASVNPILDPWIYILLRKTVLSKAIEKIKCLFCRIGGSRRERSGQHCSDSQRTSSAMSGHSRSFISRELKEISSTSQTLLPDLSLPDLSENGLGGRNLLPGVPGMGLAQEDTTSLRTLRISETSDSSQG

conjugate

unconjugated

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PTGER4(5734)

General description

PTGER4 (prostaglandin E receptor 4) is a prostanoid transmembrane receptor, which has 7 membrane spanning regions. This gene is localized to human chromosome 5p13.1. It is one of the four members of the receptors of prostaglandin E2 (PGE2), and is a G-protein coupled receptor.

Immunogen

Prostaglandin E2 receptor EP4 subtype recombinant protein epitope signature tag (PrEST)

Biochem/physiol Actions

PTGER4 (prostaglandin E receptor 4) binds to prostaglandin E2 (PGE2), and activates Gs protein, which leads to increase of intracellular cAMP levels and decrease of Ca2+ levels. Studies show that activation of this protein prevents the aggregation of platelets and has an anti-thrombotic effect. The expression of PTGER4 is enhanced by transforming growth factor β (TGF-β), which leads to an increase in PGE2-driven virulence of Theileria annulata-transformed macrophages. The SNP rs7720838 in this gene is associated with increased susceptibility to Crohn′s disease. This gene is up-regulated in lymphocytic colitis, and the expression is related with increased tumor necrosis factor-α (TNF-α) expression. PTGER4 has anti-inflammatory activity, where it inhibits the reorganization of junction and cytoskeletons of endothelial cells, promotes the healing of microvascular endothelial monolayers of lungs, and prevents the adhesion and migration of neutrophils.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST72466.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Tomoyasu Nishimura et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 27(9), 3827-3836 (2013-06-14)
Prostaglandin E2 (PGE2) is an important biological mediator involved in the defense against Mycobacterium tuberculosis (Mtb) infection. Previously, we reported that in macrophages (Mϕs), infection with avirulent Mtb H37Ra resulted in inhibition of necrosis by an inhibitory effect on mitochondrial...
Nami Shrestha Palikhe et al.
Journal of human genetics, 57(8), 494-499 (2012-06-15)
Prostaglandin E2 receptor subtype EP4 (PTGER4) is one of the four subtypes of receptors for prostaglandin E2 (PGE2). Overproduction of cysteinyl leukotriene in mast cells may be related with suppression of PGE2 in patients with aspirin hypersensitivity. Considering the association...
Jürgen Glas et al.
PloS one, 7(12), e52873-e52873 (2013-01-10)
Genome-wide association studies identified a PTGER4 expression-modulating region on chromosome 5p13.1 as Crohn's disease (CD) susceptibility region. The study aim was to test this association in a large cohort of patients with inflammatory bowel disease (IBD) and to elucidate genotypic...
Matthias Prager et al.
International journal of colorectal disease, 29(8), 909-915 (2014-05-06)
Variants modulating expression of the prostaglandin receptor 4 (PTGER4) have been reported to be associated with Cohn's disease (CD), but the clinical impact remains to be elucidated. We analyzed these variants in a large German inflammatory bowel disease (IBD) cohort...
Sonia Philipose et al.
Arteriosclerosis, thrombosis, and vascular biology, 30(12), 2416-2423 (2010-11-13)
Low concentrations of prostaglandin (PG) E(2) enhance platelet aggregation, whereas high concentrations inhibit it. The effects of PGE(2) are mediated through 4 G protein-coupled receptors, termed E-type prostaglindin (EP) receptor EP1, EP2, EP3, and EP4. The platelet-stimulating effect of PGE(2)...

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