The gene GJD2 (gap junction protein δ 2), also referred to as CX36 (connexin 36), encodes one of three major neuronal connexins, that is expressed in neurons and β-cells of the pancreas. CX36 is the only connexin expressed in β-cells of the pancreas in adults. The gene is mapped to human chromosome 15q14.
Gap junction delta-2 protein recombinant protein epitope signature tag (PrEST)
All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.
The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.
The gap junction (GJ) channels involved in electrical signaling and metabolic communication are formed by integral membrane proteins called connexins. The type of connexin influences the permeability of GJs to second messengers, such as Ca2+, cAMP and IP3. Cx36 forms GJs that are highly permeable to positively charged tracers or cations. The cells expressing Cx36 have relatively low cell-cell coupling, low single-channel conductance and low sensitivity of Vj (transjunctional voltage) gating. The β cells exchange mainly positively charged molecules with pancreatic islets via Cx36 channels. Stimulation of insulin secretion positively regulates these exchanges and Cx36 signaling participates in the modulation of insulin secretion.
Features and Benefits
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:
- IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
- Protein array of 364 human recombinant protein fragments.
Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide
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