HPA023369

Sigma-Aldrich

Anti-FAM83G antibody produced in rabbit

enhanced validation

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab1

Synonym(s):
Anti-PAWS1, Anti-FLJ41564
Human Protein Atlas Number:

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

packaging

antibody small pack of 25 μL

enhanced validation

orthogonal RNAseq
Learn more about Antibody Enhanced Validation

application(s)

immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:50-1:200

immunogen sequence

RLLPDPGSPRLAQNARPMTDGRATEEHPSPFGIPYSKLSQSKHLKARTGGSQWASSDSKRRAQ

conjugate

unconjugated

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FAM83G(644815)

General description

The gene FAM83G (family with sequence similarity 83 member G), also referred to as PAWS1 (protein associated with SMAD1), is found to be conserved across vertebrates and is a member of a family of hypothetical proteins, FAM83A–H. These proteins contain a conserved N-terminal domain called DUF1669, whose function is yet to be determined. This domain contains a putative pseudo-phospholipase D motif.

Immunogen

Protein FAM83G recombinant protein epitope signature tag (PrEST)

Biochem/physiol Actions

The gene FAM83G (family with sequence similarity 83 member G) encodes a protein that interacts with SMAD1 (similar to mothers against decapentaplegic 1). Independent of SMAD4, the encoded protein forms a complex with SMAD1 and forms a substrate for type I BMP (bone morphogenetic protein) receptor kinases. PAWS1 also forms a substrate for ALK3 (aurora-like kinase ), a type IA BMP receptor, and its phosphorylation is induced by BMPs. Phosphorylated PAWS1 regulates the expression of NEDD9 (neural precursor cell expressed, developmentally down-regulated 9 ) and ASNS (asparagine synthetase), which are SMAD4-independent BMP target genes. It also regulates the expression of several genes independent of BMP stimulation. PAWS1 is involved in the regulation of BMP pathway.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST75957.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Janis Vogt et al.
Open biology, 4, 130210-130210 (2014-02-21)
Bone morphogenetic proteins (BMPs) control multiple cellular processes in embryos and adult tissues. BMPs signal through the activation of type I BMP receptor kinases, which then phosphorylate SMADs 1/5/8. In the canonical pathway, this triggers the association of these SMADs...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.